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1983年9月,从新疆石河子豇豆病毒病植株上分离到1株病毒分离物Cp—1。汁液摩擦接种试验的结果证明,它可以侵染三种豆科植物和二种藜科植物。在豇豆上引起系统花叶,叶片皱缩,下卷并有疱疹等症状;在绿豆上表现为局部病斑。体外抗性测定,失毒温度为55—60℃,稀释限点为10~(-3)—10~(-4)。体外保毒期3—4天。Cp—1汁液易摩擦接种,桃蚜、棉黑蚜均可传毒,可通过种子传毒,种传率为10.6％。病毒粒体线条状,大小为12—5×700—750纤米。病株叶片细胞内有风轮状内含体。该分离物与豇豆蚜传花叶病毒(CA-BMV)的抗血清形成明显的沉淀线。根据以上性状,分离物Cp—1属于马铃薯y病毒组的豇豆蚜传花叶病毒。在新疆各地采取21个标样,经血清学和寄主范围测定,有13个标样属该病毒,约占62％。说明豇豆蚜传花叶病毒在新疆种植的豇豆上是普遍存在的。
In 1983 September, from Xinjiang Shihezi cowpea virus disease isolates a virus isolate Cp-1. The result of the juice friction inoculation test proved that it can infect three legumes and two Chenopodiaceae plants. On the cowpea caused by system mosaic, leaves shrunken, with volume and herpes and other symptoms; in mung bean on the performance of local lesion. In vitro resistance assay, the temperature of detoxification was 55-60 ℃, and the dilution limit was 10 -3 -3 -10 -4. In vitro protection of 3-4 days. Cp-1 juice easy to friction inoculation, Myzus persicae, cotton black aphids can be poisoned, can be poisoned by seeds, seed rate was 10.6%. Viral mitochondria, the size of 12-5 × 700-750 fiber. Diseased leaves within the wind-shaped body containing vesicles. This isolate formed a clear precipitation line with the antisera of Cowpea aphid-transmitted mosaic virus (CA-BMV). According to the above traits, isolate Cp-1 belongs to the cowpea aphid-transmitted mosaic virus of the potato y virus group. Twenty-one standard samples were taken from all over Xinjiang, and the serotypes and host range were determined. Thirteen standard samples belonged to the virus, accounting for about 62%. That cowpea aphid-transmitted mosaic virus is planted in Xinjiang cowpea ubiquitous.