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目的比较血清和EDTA抗凝血浆测定甲状旁腺素(iPTH)的稳定性以及EDTA浓度对结果的影响。方法用DPCIMMULITE测定血清和不同浓度的EDTA抗凝血浆的iPTH值,以及分别测定采血2小时内和室温放置3天的血清及EDTA抗凝血浆的iPTH值。结果EDTAK2的浓度为18mmol/L时的血浆测得的iPTH值与其余四组EDTA浓度分别为9、6、4.5、3.6mmol/L的血浆iPTH值有显著性差异;当EDTA浓度小于6mmol/L时,血浆的iPTH值与血清的值一致;采血后2小时内测得的血清iPTH与室温放置3天无显著性差异(P=0.309);采血后2小时内测得的血浆iPTH值与室温放置3天的血浆iPTH之间有显著性差异(P=0.006)。结论血浆中EDTAK2的不同浓度会造成iPTH结果的漂移,用血清测定iPTH较血浆更方便和稳定。
Objective To compare the stability of parathyroid hormone (iPTH) with serum and EDTA anticoagulated plasma and the effect of EDTA concentration on the results. Methods The iPTH values of serum and EDTA anticoagulated plasma were measured by DPCIMMULITE, and the iPTH values of serum and EDTA anticoagulated plasma were determined respectively within 2 hours after blood sampling and at room temperature for 3 days. Results Plasma iPTH values at 18 mmol / L EDTAK2 were significantly different from plasma iPTH values at 9, 6, 4.5 and 3.6 mmol / L, respectively. When EDTA concentration was less than 6 mmol / L , The iPTH value in plasma was consistent with that in serum; there was no significant difference between iPTH measured at 2 hours after blood collection and at room temperature for 3 days (P = 0.309); the plasma iPTH value measured within 2 hours after blood collection was correlated with room temperature There was a significant difference between the plasma iPTHs placed for 3 days (P = 0.006). Conclusion The different concentration of EDTAK2 in plasma can cause the drift of iPTH, and the serum iPTH is more convenient and stable than plasma.