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血药浓度的测定本文采用硅胶固相萃取柱萃取,荧光检测 HPLC 法测定人血浆中沙丁醇胺(Ⅰ)的浓度,方法快速、灵敏、准确、重现性好,适于生物利用度及药动学研究中大量样品的分析。仪器条件:固相萃取采用具50支固相硅胶萃取柱(Bond-Elut SPE)的真空多管盒。分析柱采用 Spherisorb 硅胶柱(3μm,4.6mm×10cm),柱温40℃;流动相为含0.25％乙酸铵水溶液(2mol/L,pH7.5)的甲醇溶液,流速1ml/min。荧光检测波长为225
Determination of plasma concentration In this paper, silica gel SPE extraction, fluorescence detection HPLC determination of human plasma plasma albuterol (I) concentration, rapid, sensitive, accurate, reproducible, suitable for bioavailability and Analysis of a large number of samples in pharmacokinetic studies. Instrument Conditions: Solid-phase extraction A vacuum manifold with 50 solid-phase silica gel (Bond-Elut SPE) columns was used. The column was equipped with Spherisorb silica gel column (3μm, 4.6mm × 10cm) at 40 ℃. The mobile phase consisted of a methanol solution containing 0.25% ammonium acetate (2mol / L, pH7.5) at a flow rate of 1ml / min. Fluorescence detection wavelength of 225