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以菜用大豆品系121、中科毛豆1号和普通大豆东生7号为材料,采用酶联免疫吸附法,在正常施用氮肥和磷肥的基础上,施用不同的钾肥(K0:不施钾肥;K1:K_2SO_4120 kg·hm~(-2);K2:K_2SO_4120 kg·hm~(-2)并在花期和荚期喷施1%K_2SO_4叶面肥),研究了菜用大豆与普通大豆籽粒形成期叶片中内源激素的活性变化,结果表明:开花后28~56 d菜用大豆和普通大豆叶片中内源激素IAA、GA、ZR和ABA的变化动态差异明显,其中GA和ZR的变化动态趋势相反。施用钾肥提高菜用大豆叶片中IAA、GA和ZR的含量(K2>K1>K0),降低ABA的含量(K0>K1>K2),普通大豆叶片中IAA、GA和ZR的含量对钾肥施用的响应与菜用大豆不同(K1>K2>K0),但是钾肥施用对菜用大豆和普通大豆叶片中ABA含量的影响一致(K0>K1>K2)。施钾提高了菜用大豆和普通大豆籽粒形成期叶片中IAA/ABA,GA/ABA和ZR/ABA的比值,可能是提高大豆产量和改善品质的部分生理机制。
Using vegetable soybean line 121, Zhongke soybean line No.1 and common soybean line Dongsheng 7 as materials, enzyme-linked immunosorbent assay (ELISA) was used to apply different potassium fertilizer (K0: no potassium fertilizer; K1: K_2SO_4120 kg · hm -2 and K2: K_2SO_4120 kg · hm -2, and spraying 1% K_2SO_4 foliar fertilizer at the florescence and podding stages), the effects of grain-forming period The results showed that the dynamic changes of endogenous hormones IAA, GA, ZR and ABA in vegetable soybean and common soybean leaves differed significantly from 28 to 56 days after flowering, in which GA and ZR showed the trend of dynamic change in contrast. The contents of IAA, GA and ZR (K2> K1> K0), ABA content (K0> K1> K2), IAA, GA and ZR content in common soybean leaves increased The response was different from that of vegetable soybeans (K1> K2> K0), but the effects of potash application on ABA content in vegetable and common soybean leaves were similar (K0> K1> K2). Potassium application increased the ratio of IAA / ABA, GA / ABA and ZR / ABA in the leaves of vegetable and common soybean during grain formation, which may be part of the physiological mechanism to increase soybean yield and improve quality.