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目的探讨早期生长反应基因-1( early growth response gene-1,Egr-1)mRNA在大鼠局灶性脑缺血再灌注后的表达情况。方法取10只健康雄性SD大鼠,体重200 ～250 g,应用原位杂交和RT-PCR方法检测大鼠局灶性脑缺血再灌注后Egr-1 mRNA的表达情况。结果 (1)原位杂交结果:假手术组神经元及胶质细胞为轻度阳性表达。缺血2 h再灌注2 h后,缺血侧Egr-1 mRNA细胞阳性表达明显增强。再灌注4 h时Egr-1 mRNA细胞阳性表达最高,再灌注22 h Egr-1 mRNA细胞阳性表达下降,至166 h时下降更加明显,但仍显著高于假手术组。(2)RT-PCR结果:大鼠脑缺血再灌注后缺血侧Egr-1 mRNA的表达明显高于假手术组,P<0.01。动态观察发现,脑缺血2h再灌注2 h后,Egr-1 mRNA即高表达,缺血2 h再灌注4 h达高峰,再灌注46 h已明显下降,但仍高于假手术组,P<0.01,随缺血再灌注时间延长,Egr-1 mRNA表达又逐渐增多,至再灌注166 h其表达亦明显高于假手术组,P<0.01。结论缺血再灌注后Egr-1 mRNA有规律性表达。
Objective To investigate the expression of early growth response gene-1 (Egr-1) mRNA after focal cerebral ischemia-reperfusion in rats. Methods Ten healthy male Sprague-Dawley rats weighing 200-250 g were used to detect the expression of Egr-1 mRNA after focal cerebral ischemia-reperfusion in rats by in situ hybridization and RT-PCR. Results (1) Results of in situ hybridization: The neurons and glial cells in the sham operation group were slightly positive. After 2 h of ischemia and 2 h of reperfusion, the positive expression of Egr-1 mRNA in ischemic area was significantly increased. The mRNA expression of Egr-1 was the highest at 4 h after reperfusion. The mRNA expression of Egr-1 decreased at 22 h after reperfusion, but decreased significantly at 166 h. However, it was still significantly higher than that of sham-operated group. (2) RT-PCR results: The expression of Egr-1 mRNA in the ischemic area after cerebral ischemia-reperfusion in rats was significantly higher than that in sham operation group (P <0.01). Dynamic observation showed that Egr-1 mRNA was highly expressed at 2 h after cerebral ischemia 2 h and peaked at 4 h after ischemia 2 h, but decreased significantly at 46 h after reperfusion, but still higher than that of sham operation group, P <0.01. The expression of Egr-1 mRNA gradually increased with the time of ischemia-reperfusion, and it was also significantly higher at 166 h after reperfusion than that in sham operation group, P <0.01. Conclusion Egr-1 mRNA is regularly expressed after ischemia-reperfusion.