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目的研究2-甲氧基雌二醇脂质体腹腔给药对S180腹水瘤的抑瘤效果。方法建立S180腹水瘤昆明小鼠模型,随机分为6组,每组20只,雌雄各半,分别为阴性对照组、阳性对照组、2-甲氧基雌二醇溶液组、2-甲氧基雌二醇脂质体高、中、低剂量治疗组。连续腹腔给药7 d,每2 d测量腹围,观察荷瘤鼠行为体征,7 d后每组随机抽取半数荷瘤小鼠,颈椎脱臼处死,解剖观察腹腔中主要脏器、称重,取出肝脏用中性甲醛固定,常规切片进行病理检查,收集腹水,测量体积,计算细胞存活率,流式细胞术检测肿瘤细胞周期及凋亡率的变化,剩余小鼠统计生存时间,计算生命延长率。结果 2-甲氧基雌二醇脂质体各治疗组小鼠平均生存时间明显延长。治疗组腹水量降低,腹水中癌细胞存活率下降,病理切片结果显示,癌细胞较少在腹腔内转移。结论腹腔注射2-甲氧基雌二醇脂质体可抑制S180细胞生长并减少腹水形成,对腹水引起的腹腔转移癌有治疗作用,并显示出剂量依赖性,脂质体治疗效果优于溶液组。
Objective To study the antitumor effect of intraperitoneal injection of 2-methoxyestradiol liposome on S180 ascites tumor. Methods The Kunming mouse model of S180 ascites tumor was established and randomly divided into 6 groups, 20 in each group, male and female respectively, which were negative control group, positive control group, 2-methoxyestradiol solution group, Cholesterol estradiol liposomes high, medium and low dose treatment group. The rats were sacrificed every day for 7 d, abdominal circumference was measured every 2 days, behavioral signs of tumor-bearing mice were observed. After 7 days, half of the tumor-bearing mice were randomly selected, sacrificed by cervical dislocation, dissected and observed the main organs in the abdominal cavity, The liver was fixed with neutral formalin, routine histopathological examination was performed, the ascites was collected, the volume was measured, the cell viability was calculated, the changes of tumor cell cycle and apoptosis rate were detected by flow cytometry, the remaining mice were counted for survival time, . Results The average survival time of 2-methoxyestradiol liposome treated groups was significantly prolonged. Ascites volume in the treatment group decreased, the survival rate of cancer cells in ascites decreased, and pathological sections showed that the cancer cells were less metastasized in the abdominal cavity. Conclusion Intraperitoneal injection of 2-methoxyestradiol liposomes can inhibit the growth of S180 cells and reduce the formation of ascites, which has a therapeutic effect on ascites-induced peritoneal metastases and shows a dose-dependent effect. Liposomal therapy is superior to solution group.