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BRD7基因是采用cDNA代表性差异分析法克隆得到的一个新基因 (GenBank登录号AF15 2 6 0 4 )。初步研究显示 ,它在鼻咽癌细胞和组织中表达明显下调 ,并且能部分逆转其恶性表型。在BRD7基因的编码区发现了 3个cSNP ,并且采用酵母双杂交系统筛选出 6种与BRD7蛋白相互作用的蛋白质。为进一步研究其可能的作用机制 ,通过脂质体转染方法 ,将BRD7基因导入NPC细胞株HNE1细胞中 ,采用蛋白质组技术研究该基因对鼻咽癌蛋白质表达谱的影响 ,从而考察该基因在肿瘤发生发展中的地位和作用。通过对过表达BRD7基因后鼻咽癌细胞系HNE1的蛋白质表达谱改变的研究 ,鉴定出 2 0个差异表达蛋白质 ,这些蛋白质包括精氨酸代琥珀酸裂解酶 (argini nosuccinateLyase)、金属蛋白酶抑制因子 2前体 (Metalloproteinaseinhibitor 2precursor)、蛋白酶体激活因子 2 8β亚单位(Proteaseomeactivator2 8βsubunit)、甲状腺转录因子 1(thyroidtranscriptionalfactor 1)、细胞周期蛋白H(cyclinH ,MO15 associatedprotein)等。这些差异蛋白质涉及基因表达调控、细胞周期的调控分子、细胞免疫、细胞代谢等众多事件。
The BRD7 gene is a new gene cloned using cDNA differential analysis (GenBank accession number AF15 2 6 0 4). Preliminary studies have shown that it is significantly down-regulated in nasopharyngeal carcinoma cells and tissues, and partially reverses its malignant phenotype. Three cSNPs were found in the coding region of the BRD7 gene and six proteins that interact with the BRD7 protein were selected using the yeast two-hybrid system. To further investigate its possible mechanism of action, the BRD7 gene was introduced into NPC cell line HNE1 by lipofection method, and the effect of the gene on the protein expression profile of NPC was studied by proteomic technique. The status and role of tumor occurrence and development. Twenty different proteins were identified by studying the changes in the protein expression profiles of the nasopharyngeal carcinoma cell line HNE1 after overexpression of the BRD7 gene. These proteins include argini nosuccinateLyase, metalloproteinase inhibitor 2 precursor, protease activator 2 8β subunit, thyroid transcription factor 1, and cyclinH (MO15 associated protein). These differential proteins are involved in a number of events such as gene expression regulation, cell cycle regulators, cellular immunity, cellular metabolism, and the like.