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据《中华结核和呼吸杂志》1999年11月22卷第11期报道 解放军第309医院熊志红等,为获得高效表达结核分支杆菌16000抗原的大肠杆菌工程菌,用聚合酶链反应(PCR)方法获得目的基因构建大肠杆菌高效表达菌,聚丙烯酰胺凝胶电泳分析重组蛋白抗原的相对分子质量大小及表达形式,DEAE及PEI凝胶纯化重组蛋白,western印迹及酶联免疫吸附试验分析重组蛋白的免疫原性。
According to the “Journal of Tuberculosis and Respiratory Diseases,” November 22, 1999 No. 22, No. 11 reported Xiong Zhihong 309 Hospital of PLA, in order to obtain efficient expression of Mycobacterium tuberculosis 16000 antigen Escherichia coli engineering bacteria, obtained by polymerase chain reaction (PCR) method The target gene was constructed to express E. coli and the molecular weight of the recombinant protein was analyzed by polyacrylamide gel electrophoresis. The recombinant protein was purified by gel electrophoresis (DEAE) and PEI gel, western blot and enzyme-linked immunosorbent assay Original.