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目的探讨p16基因甲基化与氡子体累积暴露剂量的关系。方法雄性Wistar大鼠20只,随机分成4组,最低剂量组暴露于普通环境中,低、中、高剂量组整体暴露于多功能生态氡室,氡子体累积暴露剂量分别达1、27、54和111工作水平月(WLM)。91名氡职业暴露工人按氡子体累积暴露剂量分为高(>120WLM)、中(60～120WLM)、低(30～59WLM)和最低(2～29WLM)4个剂量组,用聚合酶链反应-甲基化特异性(MSP)检测4组人群痰细胞和大鼠支气管上皮细胞p16基因甲基化。结果当染氡剂量达到111WLM,大鼠支气管上皮细胞出现p16基因甲基化。在铀矿工中,随着氡子体累积暴露剂量增加,痰细胞p16基因甲基化率呈上升趋势(0.00%～20.00%,P<0.01)。结论大鼠支气管上皮细胞和铀矿工痰细胞p16基因甲基化可能与氡子体累积暴露剂量有关。
Objective To investigate the relationship between methylation of p16 gene and dose of cumulative exposure to radon. Methods Twenty male Wistar rats were randomly divided into 4 groups. The lowest dose group was exposed to normal environment. The low, medium and high dose groups were exposed to the multifunctional radon chamber. The cumulative exposure dose of radon was 1,27, 54 and 111 working-level months (WLM). Ninety-one exposed workers exposed to radon were divided into 4 groups (high (> 120WLM), moderate (60-120WLM), low (30-59WLM) and lowest (2-29WLM) Methylation of p16 gene in sputum cells and rat bronchial epithelial cells was detected by response-methylation-specific (MSP) assay. Results When the dose of radon reached 111WLM, p16 gene methylation occurred in the bronchial epithelial cells of rats. In uranium miners, the methylation rate of p16 gene in sputum cells showed an upward trend (0.00% -20.00%, P <0.01) with the cumulative dose of radon progeny increasing. Conclusion Methylation of p16 gene in rat bronchial epithelial cells and uranium miners’ sputum cells may be related to the cumulative dose of radon progenitors.