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目的:利用D-半乳糖引起小鼠衰老模型,观察灯盏花素(Breviscapine,Bre)对衰老小鼠记忆障碍、肝肾组织结构异常改变及血脂代谢的影响。方法:雌性昆明小鼠60只随机分为5个组,除空白对照组外,其他各组小鼠每天皮下注射D-半乳糖120mg/kg制备衰老模型,从第41d开始,3个剂量药物组小鼠在sc D-半乳糖的同时,腹腔注射Bre 5,10和20 mg/kg 67d,在给药第66d进行避暗箱实验训练,24h后进行小鼠记忆能力检测,在给药第67d避暗实验结束后小鼠断颈处死,取血清测定血脂变化,分离肝脏和肾脏HE染色观察其组织结构变化。结果:D-半乳糖明显引起小鼠记忆障碍、肝肾组织结构异常和血脂紊乱。与空白对照组相比,模型组小鼠避暗实验潜伏期缩短(P<0.01)、受电击次数多(P<0.01),血清中甘油三酯(TG)、总胆固醇(TC)和低密度脂蛋白(LDL-C)升高(P<0.01)、高密度脂蛋白(HDL-C)降低(P<0.05),肝、肾组织结构异常改变。5,10和20mg/kg灯盏花素给药67天可不同程度地降低D-半乳糖引起小鼠避暗实验潜伏期缩短和受电击次数的增多,改善血清血脂水平和肝、肾组织结构异常变化。结论:灯盏花素对D-半乳糖引起小鼠记忆障碍、血脂紊乱及肝肾组织结构异常改变具有明显的改善作用,该作用提示灯盏花素可能有利于延缓衰老和老年性痴呆病的治疗。
OBJECTIVE: To observe the effects of Breviscapine (Bre Breastcapine) on memory impairment, abnormal changes of hepatic and renal tissues and lipid metabolism in mice induced by D-galactose. Methods: Sixty female Kunming mice were randomly divided into five groups. Except for the blank control group, the other groups of mice were injected subcutaneously with D-galactose 120mg / kg to prepare the aging model. From the 41st day, the three dose groups Mice were injected intraperitoneally with Bre 5, 10 and 20 mg / kg 67d at the same time with sc D-galactose, darkroom experiments were conducted on the 66th day after administration, memory ability of mice was detected 24 hours later, After dark experiment, the mice were sacrificed by cervical dislocation, the changes of serum lipid were measured, and the changes of liver and kidney were observed by HE staining. Results: D-galactose significantly induced memory impairment, structural abnormalities in liver and kidney and dyslipidemia. Compared with the blank control group, the mice in the model group had a shorter latency to avoid darkness (P <0.01), the number of shocks (P <0.01), serum triglyceride (TG), total cholesterol (TC) and low density lipids (LDL-C) increased (P <0.01), HDL-C decreased (P <0.05), and the structure of liver and kidney changed abnormally. The administration of 5, 10, and 20 mg / kg Breviscapine for 67 days reduced the darkening latency and the number of shocks induced by D-galactose in mice, and improved the level of serum lipid and the abnormal changes of liver and kidney tissues . Conclusion: Breviscapine can significantly improve memory impairment, dyslipidemia and abnormal structure of liver and kidney in D-galactose-induced mice, which suggests that Breviscapine may be beneficial to delay aging and senile dementia.