最近我在预备变形虫实验过程中,曾遇到了这样的困难(也许是技术水平不够)即是无法很好地在双管解剖镜下用微吸管吸去轮虫、纤毛虫等对变形虫繁殖不利的原生动物,其结果就影响了变形虫的培养工作。后来我改用了下列方法,认为效果良好,手续简便,颇适用于一般中学及大学普通生物学准备变形虫实验工作时之参考,兹特介绍如下:(1)采集:据我们几年来的经验,春秋季节在武昌常可较不费力地自荷塘里采到变形虫,其方法是在荷塘边沿浅处,把腐烂荷叶连杂草带污泥采入玻缸中,再加上塘水少许带回,特别要指出的是上述采集物常具有腐臭、不像普通塘水之清澈(因污泥或腐败之植物所致)。(2)培养:首先,将采集物静置一两天,然后用滴管自缸边沿吸取少许 Recently, I encountered such difficulties (perhaps not enough technical skills) during the preparation of the amoeba experiment that I was unable to properly suck rotifera with a micropipette under a double-tube dissecting microscope and ciliates propagate the amoeba Adverse protozoa, the result has affected the cultivation of amoeba. Later, I use the following method, that the effect is good, the procedure is simple, quite suitable for general secondary school and university general biology preparation for the experimental work Amoeba reference, hereby introduced as follows: (1) Collection: According to our years of experience Spring and autumn seasons in Wuchang often can be more effortless to pick the amoeba from the pond, the method is in the shallow edge of the lotus pond, the decay lotus leaf with weeds and sludge taken into the tank, together with a little pond water Back, in particular, pointed out that the above-mentioned collection often has rancid, unlike ordinary pond water clear (due to sludge or corrupt plants caused). (2) Cultivation: First, collect the collected material for a day or two, and then use the dropper to draw a little from the edge of the cylinder