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目的: 了解新型抑癌基因p16 在人卵巢上皮癌细胞系中的变异情况, 为认识卵巢上皮癌的形成和发展提供依据。方法: 应用PCR 扩增、m RNA 原位杂交和免疫细胞化学方法, 对5 种人卵巢上皮癌细胞系CAOV3 、OVCAR3 、AO、HO- 8910 及HO- 8910PM 进行分析。结果:5 种人卵巢上皮癌细胞系中只有CAOV3(20% ) 显示p16 基因纯合性缺失,CAOV3 细胞亦无p16 基因m RNA 及蛋白表达。OVCAR3 、HO- 8910 及HO- 8910PM 呈p16 m RNA 杂交阳性反应及免疫组化阳性反应,阳性颗粒主要分布在癌细胞浆内。AO 细胞虽有p16 m RNA 表达,却无p16蛋白表达。结论:本组p16 基因的分析结果为人卵巢上皮癌细胞系第9 号染色体基因变异及卵巢上皮癌的形成和发展提供了新理论依据。
OBJECTIVE: To understand the variation of the novel tumor suppressor gene p16 in human ovarian epithelial carcinoma cell lines, and to provide a basis for understanding the formation and development of epithelial ovarian cancer. Methods: Five human epithelial ovarian cancer cell lines, CAOV3, OV-CAR3, AO, HO-8910 and HO-8910PM, were analyzed by PCR amplification, m RNA in situ hybridization and immunocytochemistry. RESULTS: Only CAOV3 (20%) showed homozygous deletion of p16 gene in 5 human epithelial ovarian cancer cell lines and no p16 m RNA and protein expression in CAOV3 cells. OVCAR3, HO-8910 and HO-8910PM positive p16 m RNA hybridization and immunohistochemical positive reaction, the positive particles are mainly distributed in the cytoplasm of cancer cells. Although AO cells expressed p16mRNA, there was no p16 protein expression. Conclusion: The results of p16 gene analysis in this study provide a new theoretical basis for the genetic variation of chromosome 9 and the formation and development of epithelial ovarian cancer in human ovarian epithelial carcinoma cell line.