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研究了采用高效液相色谱 紫外检测法对盐酸赖氨酸注射液中赖氨酸含量的测定 .作者在AminoQuant方法的基础上 ,改为仅采用邻苯二醛 (OPA)衍生化 ,省略了原方法衍生化试剂中的不稳定成分 ,而采用优化的反应条件提高紫外测定的灵敏度 ;又对这个组成较简单的样品改用恒定组成的流动相淋洗 ,手动方法测定避免了对该试样不必要的梯度淋洗 ,较原AminoQuant氨基酸分析法简便、快速 ,结果峰面积与浓度相关系数r =0 9998,回收率为 99 7% (RSD为 1 0 %～ 1 5 % ) .在采用紫外检测法时 ,最小检出量为 5 3× 10 -8(S/N =3)与原方法相近
The determination of lysine in lysine hydrochloride injection by high performance liquid chromatography with ultraviolet detection was studied in this paper.On the basis of AminoQuant method, only the derivative of ortho-phthalaldehyde (OPA) was used instead of the original Method to derivatize the unstable components of the reagent while using the optimized reaction conditions to increase the sensitivity of the UV measurement; again, the simpler sample was switched to a constant composition mobile phase elution, and the manual method was used to avoid The required gradient elution was simpler and faster than the original AminoQuant amino acid analysis, with a correlation coefficient of peak area to concentration of r = 0 9998 and a recovery of 99.7% (RSD 10% ~ 15%). After UV detection Method, the minimum detection capacity of 5 3 × 10 -8 (S / N = 3) is similar to the original method