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目的:探讨趋化因子受体CCR6、CCR7及其配体CCL20、CCL19/CCL21在喉鳞状细胞癌(LSCC)中的表达及其与临床病理特征的相关性。方法:以50例LSCC患者的肿瘤组织、癌旁组织、颈清扫淋巴结、外周血为研究对象,采用实时定量逆转录聚合酶链反应(real-timeqRT-PCR)技术、免疫组织化学技术及流式细胞术检测趋化因子受体CCR6、CCR7及其配体(CCL20、CCL19/CCL21)的表达。结果:real-timeqRT-PCR检测发现LSCC肿瘤组织中CCR6、CCR7及CCL19/CCL21mRNA的表达量低于癌旁组织(P<0.05),CCL20mRNA的表达量高于癌旁组织(P<0.05);免疫组织化学检测发现LSCC肿瘤组织及转移淋巴结均有CCR6和CCR7的表达,且伴颈淋巴结转移的LSCC组织CCR6、CCR7的表达高于不伴颈淋巴结转移组织,差异有统计学意义(P<0.05);流式细胞术检测发现,LSCC患者外周血CD4+CCR6+T细胞占外周血单个核细胞的比例显著高于正常对照组(P<0.05),而CD4+CCR7+T细胞的比例则显著低于正常对照组(P<0.05)。结论:趋化因子受体CCR6、CCR7在LSCC组织、转移淋巴结及外周血中表达,与LSCC的发展、浸润、颈部淋巴结转移有关。
Objective: To investigate the expression of chemokine receptors CCR6, CCR7 and their ligands CCL20 and CCL19 / CCL21 in laryngeal squamous cell carcinoma (LSCC) and their relationship with clinicopathological features. Methods: The tumor tissues, pericarcinomatous tissues, cervical lymph nodes and peripheral blood of 50 patients with LSCC were studied by real-time qRT-PCR, immunohistochemistry and flow cytometry Cytometry was used to detect the expression of chemokine receptors CCR6, CCR7 and their ligands (CCL20, CCL19 / CCL21). Results: The expression of CCR6, CCR7 and CCL19 / CCL21 mRNA in LSCC was lower than that in adjacent non-cancerous tissues (P <0.05) by real-time qRT-PCR. The expression of CCL20 mRNA was higher than that in paracancerous tissues The expression of CCR6 and CCR7 in both LSCC and metastatic lymph nodes were found by histochemical detection. The expressions of CCR6 and CCR7 in LSCC with lymph node metastasis were significantly higher than those without lymph node metastasis (P <0.05) Flow cytometry showed that the percentage of CD4 + CCR6 + T cells in peripheral blood mononuclear cells in peripheral blood was significantly higher in LSCC patients than that in normal controls (P <0.05), while the proportion of CD4 + CCR7 + T cells was significantly lower in LSCC patients In normal control group (P <0.05). Conclusion: The chemokine receptors CCR6 and CCR7 are expressed in LSCC tissues, metastatic lymph nodes and peripheral blood, which are related to the development of LSCC, infiltration and lymph node metastasis.