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目的:研究Th17和Treg细胞以及其相关细胞因子在慢性丙型肝炎以及丙型肝炎肝硬化病人外周血中的变化及意义,以探讨其在慢性丙型肝炎及丙型肝炎肝硬化发病中的作用。方法:用流式细胞和ELSIA技术检测慢性丙型肝炎及丙型肝炎肝硬化病人外周血Th17和Treg细胞表达率以及IL-6、IL-10、TGF-β、IL-17血清学水平,分析上述指标在健康对照组和慢性丙型肝炎组以及肝硬化组之间的差异。结果:慢性丙型肝炎病人的Th17细胞表达率(1.33%±0.30%)以及IL-6[(8.10±2.42)ng/L]、IL-17[(16.70±4.73)ng/L]血清水平明显高于健康对照组Th17细胞表达率(1.14%±0.19%)以及IL-6[(6.70±1.72)ng/L]、IL-17[(12.29±1.88)ng/L]血清水平,P<0.05;丙型肝炎肝硬化组和慢性丙型肝炎组病人外周血的Treg细胞表达率(6.21%±0.76%,5.89%±0.85%)明显高于健康对照组的Treg细胞表达率(5.51%±0.59%),P<0.05;丙型肝炎肝硬化组病人Th17/Treg的比值(0.19±0.02)明显低于慢性丙型肝炎组(0.22±0.03)和健康对照组(0.21±0.03),P<0.05;丙型肝炎肝硬化组外周血IL-10水平[(16.21±3.76)ng/L]和TGF-β水平[(5.15±0.83)ng/L]显著高于慢性丙型肝炎组[(14.36±2.78)ng/L;(4.47±0.87)ng/L]和健康对照组[(14.01±3.01)ng/L;(4.43±0.98)ng/L],P<0.05。结论:Th17和Treg细胞以及其相关细胞因子参与了丙型肝炎慢性化和肝硬化的进程,Th17和Treg细胞以及其相关细胞因子在丙型肝炎慢性化和肝硬化的治疗和预防方面可能具有重要的意义。
Objective: To investigate the changes and significance of Th17 and Treg cells and related cytokines in peripheral blood of patients with chronic hepatitis C and hepatitis C cirrhosis so as to investigate their roles in the pathogenesis of chronic hepatitis C and hepatitis C cirrhosis . Methods: Serum levels of Th17 and Treg cells, IL-6, IL-10, TGF-β and IL-17 in peripheral blood of patients with chronic hepatitis C and hepatitis C cirrhosis were detected by flow cytometry and ELSIA. The above mentioned differences between healthy control group and chronic hepatitis C group and cirrhosis group. Results: The serum levels of Th17 cells in chronic hepatitis C (1.33% ± 0.30%) and IL-6 [(8.10 ± 2.42) ng / L] and IL-17 [(16.70 ± 4.73) ng / L] (1.14% ± 0.19%) and IL-6 [(6.70 ± 1.72) ng / L] and IL-17 [(12.29 ± 1.88) ng / L] (6.21% ± 0.76%, 5.89% ± 0.85%) in peripheral blood of patients with hepatitis C cirrhosis and chronic hepatitis C were significantly higher than that of healthy control (5.51% ± 0.59 %), P <0.05. The ratio of Th17 / Treg in patients with hepatitis C cirrhosis was significantly lower than that in patients with chronic hepatitis C (0.22 ± 0.03) and healthy controls (0.21 ± 0.03), P <0.05 ; The levels of IL-10 in peripheral blood of patients with hepatitis C cirrhosis [(16.21 ± 3.76) ng / L] and TGF-β levels (5.15 ± 0.83) ng / L were significantly higher than those in patients with chronic hepatitis C [(14.36 ± 2.78) ng / L; (4.47 ± 0.87) ng / L], and healthy control group [(14.01 ± 3.01) ng / L and (4.43 ± 0.98) ng / L], respectively. CONCLUSION: Th17 and Treg cells and their associated cytokines are involved in the progression of chronic hepatitis C and cirrhosis. Th17 and Treg cells and their associated cytokines may be important in the treatment and prevention of chronic hepatitis C and cirrhosis Meaning.