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目的调查健康人和冠状动脉粥样硬化性心脏病(简称冠心病)患者的纤维蛋白原B(fibrino-genB,FGB)β基因-1420G/A、-993C/T和-854G/A的基因多态性频率分布,以及与血浆Fg水平的关系。方法应用等位基因特异性PCR扩增技术和限制性片段长度多态性技术对186例冠心病患者和149名健康对照者进行分析。比浊法测定血浆Fg浓度。结果等位基因频率-1420A在冠心病组为0.33,在对照组为0.26,冠心病组明显比对照组升高,两者比较差异有统计学意义(P<0.05)。等位基因频率-993T和-854A在两组之间无差别。Logistic回归分析显示β-1420G/A与冠心病相关(OR=1.922,P=0.003)。冠心病组血浆Fg水平(3.87±1.75)g/L较健康人组(3.10±0.77)g/L明显升高,且差异有统计学意义(P<0·05)。结论纤维蛋白原Bβ-1420G/A可能与冠心病发病相关联。
Objective To investigate the gene polymorphisms of -1420G / A, -993C / T and -854G / A of fibrinogen-genB (FGB) β gene in healthy individuals and patients with coronary atherosclerotic heart disease The frequency of the state, and the relationship with plasma Fg levels. Methods A total of 186 patients with coronary heart disease and 149 healthy controls were analyzed using allele-specific PCR and restriction fragment length polymorphism (PCR-RFLP). Turbidimetric determination of plasma Fg concentration. Results The frequency of allele-1420A was 0.33 in CHD group and 0.26 in control group, which was significantly higher in CHD group than that in control group (P <0.05). Allele frequencies -993T and -854A did not differ between the two groups. Logistic regression analysis showed that β-1420G / A was associated with coronary heart disease (OR = 1.922, P = 0.003). The level of plasma Fg in coronary heart disease group (3.87 ± 1.75) g / L was significantly higher than that in healthy people (3.10 ± 0.77) g / L, and the difference was statistically significant (P <0.05). Conclusion Fibrinogen Bβ-1420G / A may be associated with coronary heart disease.