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将丙型肝炎病毒Ｃ＋Ｅ１区基因插入到原核高效表达载体ｐＢＶ２２１质粒中，构建了质粒ｐＢＶ２２１ＨＣＶ／Ｃ＋Ｅ１作为表达载体，然后，将含有该质粒的宿主大肠杆菌进行升温诱导表达ＨＣＶ／Ｃ＋Ｅ１区基因，并对表达产物进行了生物活性的检测。结果表明，插入到表达载体ｐＢＶ２２１中的ＨＣＶ／Ｃ＋Ｅ１基因片段能够得到有效的表达，表达产物主要为非融合蛋白形式存在于细胞中，同时这种Ｃ区和Ｅ１区连接共表达的产物保持了良好的抗原活性
The C + E1 region of hepatitis C virus was inserted into the prokaryotic expression vector pBV221, and the plasmid pBV221 HCV / C + E1 was constructed as an expression vector. Then, the host E. coli containing the plasmid was induced to heat up to express HCV / C + E1 region, The expression product was tested for biological activity. The results showed that the HCV / C + E1 gene fragment inserted into the expression vector pBV221 was able to express efficiently, and the expression product was mainly in the form of non-fusion protein, and the co-expression product of C region and E1 region maintained good Antigenic activity