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目的：研究淫羊藿甙（ＩＣＡ）对ＰＧ细胞分泌转化生长因子β２（ｔｒａｎｓｆｏｒｍｉｎｇ ｇｒｏｗｔｈ ｆａｃｔｏｒβ２，ＴＧＦβ２）的影响、ＩＣＡ逆转ＴＧＦβ２介导的免疫抑制作用及其机制。方法：ＭＴＦ法检测细胞增殖、杀伤活性，ＲＴ－ＰＣＲ检测ＴＧＦβ２、穿孔素，流式细胞术检测ＴＧＦβ２、ＩＬ－２Ｒα水平。结果：ＩＣＡ可抑制ＰＧ细胞中ＴＧＦβ２的蛋白和ｍＲＮＡ表达，能够逆转受ＴＧＦβ２抑制的ＬＡＫ和ＣＤ３ＡＫ细胞的杀伤活性，并能部分恢复受ＴＧＦβ２抑制的ＬＡＫ细胞表面ＩＬ－２Ｒα表达和ＣＤ３ＡＫ细胞内穿孔素ｍＲＮＡ水平，以及ＣＤ３ＡＫ细胞的增殖活性。结论：淫羊藿甙通过抑制肿瘤细胞免疫抑制因子ＴＧＦβ２的产生，增强免疫效应细胞的杀伤活性等机制而发挥抗肿瘤作用。
Objective: To study the effect of epimedium (ICA) on the secretion of transforming growth factor β2 (TGFβ2) in PG cells and the mechanism of ICA reversing the immunosuppression induced by TGFβ2. Methods: The cell proliferation and killing activity were detected by MTT assay. TGFβ2 and perforin were detected by RT-PCR. The levels of TGFβ2 and IL-2Rα were measured by flow cytometry. RESULTS: ICA inhibited the expression of TGFβ2 protein and mRNA in PG cells, reversed the cytotoxic activity of TGFβ2 inhibiting LAK and CD3AK cells, and partially restored the expression of IL-2Rα on the surface of LAK cells inhibited by TGFβ2 and perforin in CD3AK cells. mRNA levels, and proliferation activity of CD3AK cells. Conclusion: Epimedium exerts anti-tumor effect by inhibiting the production of tumor cell immunosuppressive factor TGFβ2 and enhancing the killing activity of immune effector cells.