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目的：分析乙型肝炎病毒（ＨＢＶ）前Ｃ区基因变异在肝纤维化形成中的作用，探讨肝纤维化与一氧化氮的关系。方法：利用错配ＰＣＲ限制片段多态性分析，检测ＨＢＶ前Ｃ区１８９６位点突变，并检测慢性乙肝患者血清Ⅳ型胶原／层粘连蛋白（ＣⅣ／ＬＮ）和ＮＯ。结果：Ａ组３１例前Ｃ区变异或变异优势患者ＣⅣ（１２７．３５±２４．３３）μｇ／Ｌ，ＬＮ（１６９．１０±２８．２３）μｇ／Ｌ明显高于Ｂ组３０例无前Ｃ区基因变异患者ＣⅣ（８６．８４±１２．６６）μｇ／Ｌ，ＬＮ（９４．２０±１５．２９）μｇ／Ｌ（Ｐ＜０．００１），且Ａ组ＮＯ－２／ＮＯ－３（４８．５５±４．０３）μｍｏｌ／Ｌ明显高于Ｂ组ＮＯ－２／ＮＯ－３（３６．６０±４．０２）μｍｏｌ／Ｌ（Ｐ＜０．００１）。结论：ＨＢＶ前Ｃ区变异与肝纤维化、肝硬化发生发展有关系，且血清ＮＯ随ＣⅣ和ＬＮ增高而增高。
Objective: To analyze the role of pre-C gene mutation in hepatitis B virus (HBV) in the formation of hepatic fibrosis and to explore the relationship between hepatic fibrosis and nitric oxide. Methods: Mismatch PCR restriction fragment polymorphism analysis was used to detect the 1896 site mutation in pre-HBV precore and to detect the serum level of type Ⅳ collagen / laminin (C Ⅳ / LN) and NO in chronic hepatitis B patients. Results: The CⅣ (127.35 ± 24.33) μg / L and LN (169.10 ± 28.23) μg / L were significantly higher in group A than in group B C (86.84 ± 12.66) μg / L and LN (94.20 ± 15.29) μg / L (P <0.001) in patients with C gene mutation, and NO-2 / NO-3 (48.55 ± 4.03) μmol / L was significantly higher than that of NO-2 / NO-3 (36.60 ± 4.02) μmol / L in group B (P <0.001). Conclusion: The pre-HBV C mutation is related to the occurrence and development of hepatic fibrosis and cirrhosis, and serum NO increases with the increase of CⅣ and LN.