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目的:建立测定7种药用黄芩中黄芩苷和汉黄芩苷的反相高效液相色谱法,7种药用黄芩为黄芩,粘毛黄芩,甘肃黄芩,滇黄芩,韧黄芩,川黄芩和丽江黄芩。方法:采用Wakosil;C_(18)柱(150mm×4.6mm,5μm),以甲醇-水-冰乙酸(41:59:0.2)和(36:64:0.2)为流动相,流速为1.0mL·min~(-1),检测波长为280nm。应用二极管阵列检测器对峰的纯度进行鉴定。结果:样品中黄芩苷和汉黄芩苷平均回收率分别为98.3％和96.8％,RSD分别为0.48％和1.3％,n=5;黄芩苷在0.08～0.4μg内,r=0.9998,汉黄芩苷在0.04～0.2 μg内,r=0.9995;峰面积与浓度线性关系良好。结论:黄芩苷、汉黄芩苷与其他成分能得到很好的分离,不同品种间2种苷含量差别比较大。
OBJECTIVE: To establish a RP-HPLC method for the determination of baicalin and crotonin in 7 kinds of medicinal baicalin. Seven kinds of medicinal yellow baicalin are Astragalus membranaceus, Visccus villosa, Astragalus membranaceus, Astragalus membranaceus, Rhizoma astragali, Rhizoma Astragali and Lijiang. Huang Hao. Method: Wakosil; C 18 column (150mm×4.6mm, 5μm) was used with methanol-water-glacial acetic acid (41:59:0.2) and (36:64:0.2) as mobile phase, and the flow rate was 1.0mL. Min~(-1), detection wavelength is 280 nm. A diode array detector was used to identify peak purity. Results: The average recoveries of baicalin and wogonin in the samples were 98.3% and 96.8%, RSD was 0.48% and 1.3%, n=5, baicalin in 0.08-0.4μg, r=0.9998, wogonin Within 0.04 to 0.2 μg, r=0.9995; the linear relationship between peak area and concentration is good. Conclusion: Baicalin, crotonin and other components can be well separated, and there is a great difference in the content of two kinds of glycosides among different varieties.