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目的观察南瓜蛋白(CUS)对人胰腺癌细胞系PANC-1的细胞毒性和化疗增敏作用。方法采用四甲基偶氮唑蓝(MTT)比色法,观察不同浓度(量效)的CUS及作用不同时间(时效)对胰腺癌细胞PANC-1的细胞毒性;采用Chou Talalay联合指数法判断CUS增加胰腺癌细胞对吉西他滨(GEM)的化疗敏感性。结果CUS对胰腺癌PANC-1细胞具有显著的增殖抑制作用,0.3125,0.625,1.25,2.5,5.0,10.0,20.0,40.0,80.0μg/mL的CUS作用于胰腺癌细胞24 h,其抑制率分别为9.16%,11.9%,14.4%,20.2%,32.0%,36.4%,44.1%,50.3%和58.6%,呈现明显的剂量依赖性;随着作用时间的延长,其抑制率明显增加,24,48,72 h的半数抑制浓度(IC50)分别为40.24,8.24,1.17μg/mL,呈现明显的时间依赖性。两药在不同效应时各自所需浓度之间相互作用均为协同作用(CI<1),单吉西他滨用于胰腺癌细胞的IC50为36.76 nmol/L,CUS与其合用时的IC50为12.14 nmol/L,合用比单用时吉西他滨的剂量减少67.0%。结论CUS对胰腺癌的细胞毒性作用呈明显的剂量依赖性和时间依赖性,与吉西他滨联合使用可增加其细胞毒作用,起到化疗增敏效应。
Objective To observe the cytotoxicity and chemosensitivity of pumpkin protein (CUS) to human pancreatic cancer cell line PANC-1. Methods Methyl thiazolyl tetrazolium (MTT) colorimetric assay was used to observe the cytotoxicity of CUS at different dosages (dose-effect) and the effect of different time (aging) on PANC-1 in pancreatic cancer cells. Chou Talalay’s combined index CUS increases the chemosensitivity of pancreatic cancer cells to gemcitabine (GEM). Results CUS had a significant inhibitory effect on pancreatic cancer PANC-1 cells. CUS at 0.3125, 0.625, 1.25, 2.5, 5.0, 10.0, 20.0, 40.0 and 80.0 μg / mL was applied to pancreatic cancer cells for 24 h, Which was significantly higher than that of the control group (P <0.05). The inhibitory rate was significantly higher than that of the control group (9.16%, 11.9%, 14.4%, 20.2%, 32.0%, 36.4%, 44.1%, 50.3% and 58.6% The IC50 values at 48 and 72 h were 40.24, 8.24 and 1.17 μg / mL, respectively, showing a significant time-dependent manner. The interaction between the two drugs in different concentrations was synergistic (CI <1). The IC50 of single gemcitabine for pancreatic cancer cells was 36.76 nmol / L, and the IC50 of CUS when combined with them was 12.14 nmol / L , The combined use of gemcitabine dose reduction of 67.0%. Conclusion CUS has a dose-dependent and time-dependent cytotoxic effect on pancreatic cancer. Combined with gemcitabine, CUS can increase its cytotoxicity and play a chemosensitizing effect.