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目的观察 ATRA,5-Fu 单独和联合应用对体外培养的胃癌细胞端粒酶活性及细胞生长的影响.方法分别用 ATRA,5-Fu 单独和联合处理胃癌 MGC-803细胞,采用 MTT 法测定细胞活力,采用端粒重复序列扩增法测定端粒酶活性.结果胃癌细胞活力随 ATRA,5-Fu 浓度增高、作用时间的延长其细胞活力逐渐下降,ATRA d1,d3,d5的 IG_(50)分别为>40μmol/L,(20～40)μmol/L,(10～20)μmol/L;5-Fu d1,d3,d 5的 IC_(50)分别为>25/μmol/L,(2～5)/μmol/L,(2～5)μmol/L;40μmol/L ATRA,5μmol/L 5-Fu 及40μmol/LATRA+5μmol/L 5-Fu 处理胃癌细胞3 d 其细胞活力分别为46%,47%,8%(P<0.01),端粒酶活性分别为45.68%(P<0.01),100.00%,46.10%(P<0.01).结论 ATRA,5-Fu 均能抑制胃癌细胞的生长,其抑制作用具有时间和浓度依赖性,且二者合用具有协同抑制作用;ATRA能抑制胃癌细胞端粒酶活性,而5-Fu 对胃癌细胞端粒酶活性无影响,二者合用对胃癌细胞端粒酶活性无协同抑制作用.抑制端粒酶活性可能是 ATRA 抗癌机制之一.
Objective To observe the effects of ATRA and 5-Fu alone and in combination on the telomerase activity and cell growth of gastric cancer cells cultured in vitro. METHODS: MGC-803 cells were treated with ATRA and 5-Fu alone and in combination, and cells were measured by MTT assay. Vitality, telomerase activity was measured by telomere repeat amplification. Results The cell viability of gastric cancer cells increased with the increase of ATRA and 5-Fu concentrations and the prolongation of the action time. The cell viability gradually decreased. The IG_(50) of ATRA d1, d3, d5 They were >40 μmol/L, (20–40) μmol/L, (10–20) μmol/L, respectively; the IC_(50) of 5-Fu d1, d3, and d 5 were >25/μmol/L, respectively (2 (5)/μmol/L, (2~5) μmol/L; 40μmol/L ATRA, 5μmol/L 5-Fu and 40μmol/LATRA+5μmol/L 5-Fu treatment of gastric cancer cells for 3 d %, 47%, 8% (P<0.01), telomerase activity were 45.68% (P<0.01), 100.00%, 46.10% (P<0.01). Conclusion ATRA, 5-Fu can inhibit gastric cancer cells The inhibition of growth was time- and concentration-dependent, and the combination of them had a synergistic inhibitory effect; ATRA could inhibit the telomerase activity of gastric cancer cells, but 5-Fu had no effect on the telomerase activity of gastric cancer cells, and the combination of them could be used for gastric cancer. Cell telomerase activity without synergistic inhibition Inhibition of telomerase activity may be one of the anti-cancer mechanisms of ATRA.