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目的： 对比研究多巴胺１（ＤＡ１） 和多巴胺２（ＤＡ２） 受体激动剂对兔冠状动脉和肾动脉ｃＡＭＰ生成量的影响。方法： 以ｃＡＭＰ生成量为生化指标，观察了ＤＡ１ 及ＤＡ２ 受体激动剂对兔冠状动脉及肾动脉ＡＣ活性的影响。结果： 发现ＤＡ１ 受体激动剂Ｆｅｎｏｌｄｏｐａｍ（ＦＯＤＡ） 及ＤＡ２ 受体激动剂ＮｎｐｒｏｐｙｌＮｎｂｕｔｙｌｄｏｐａｒｎｉｎｅ（ＰＢＤＡ） 均可剂量依赖性增加冠状动脉及肾动脉ｃＡＭＰ的生成量。然而，肾动脉ｃＡＭＰ的生成量均显著高于冠状动脉ｃＡＭＰ 的生成量，选择性ＤＡ１ 受体阻断剂ＳＣＨ２３３９０ 能够阻断ＦＯＤＡ及ＰＢＤＡ所引起的ｃＡＭＰ生成量的增加，而ＤＡ２ 受体阻断剂ｄｏｍｐｅｒｉｄｏｎｅ 则对ＰＢＤＡ 的这一作用没有影响。心得安阻断β受体后，ＦＯＤＡ仍可显著增加ｃＡＭＰ的生成量，但增加的程度却明显降低。结论： 兔肾动脉及冠状动脉上都存在有刺激ＡＣ活性的ＤＡ１ 受体，但冠状动脉ＤＡ１ 受体的位点数比肾动脉ＤＡ１ 位点数要少得多；冠状动脉ＤＡ１ 受体的作用比肾动脉要弱。
Objective: To compare the effects of dopamine 1 (DA1) and dopamine 2 (DA2) receptor agonists on cAMP production in the coronary and renal arteries of rabbits. Methods: The cAMP production was taken as a biochemical index to observe the effects of DA1 and DA2 receptor agonists on the AC activity of the coronary and renal arteries in rabbits. RESULTS: DA1 receptor agonist Fenoldopam (FODA) and DA2 receptor agonist NnpropylNbutyldoparnine (PBDA) were found to increase cAMP production in both coronary and renal arteries in a dose-dependent manner. However, the production of cAMP in renal arteries was significantly higher than that of coronary cAMP. The selective DA1 receptor blocker SCH23390 blocked the increase of cAMP production induced by FODA and PBDA, while DA2 receptor blockers domperidone has no effect on this role of PBDA. Adiponectin beta blocker, FODA can still significantly increase the formation of cAMP, but the extent of the increase was significantly reduced. CONCLUSION: There are DA1 receptors on rabbit renal artery and coronary arteries which stimulate AC activity. However, the number of DA1 receptors in coronary artery is much less than that of DA1 in renal artery. The effect of DA1 receptor on coronary artery is lower than that of renal artery To be weak.