钙调素(CaMs,calmodulins)是一类非常保守的Ca2+感受蛋白,在Ca2+信号转导中起重要调节作用。本研究分析了强抗逆植物沙冬青(Ammopiptanthus mongolicus)AmCaM1在非生物胁迫下的表达变化,克隆了该基因并将其构建到植物表达载体上,然后转化拟南芥进行了初步的功能分析。结果表明,AmCaM1的转录水平在低温、干旱和盐胁迫下迅速上调;其cDNA编码区由450 bp组成,编码蛋白含149个氨基酸残基,在其一级结构中具有4个保守的EF-手型基序;将该基因转化拟南芥可提高种子萌发期对水分胁迫的耐性,而对耐盐和耐冷性无明显作用。 Calmodulins (CaMs) are a kind of very conserved Ca2 + sensing protein that plays an important regulatory role in Ca2 + signal transduction. In this study, the expression of AmCaM1, a strong resistance plant, was analyzed under abiotic stress. The gene was cloned and then constructed into a plant expression vector, and then transformed into Arabidopsis thaliana for preliminary functional analysis. The results showed that the transcription level of AmCaM1 was rapidly up-regulated under the conditions of low temperature, drought and salt stress. The cDNA coding region consisted of 450 bp and contained 149 amino acid residues and four conserved EF-arms in its primary structure Type motif. The transformation of Arabidopsis thaliana into Arabidopsis could improve the tolerance to water stress at seed germination, but had no significant effect on salt tolerance and cold tolerance.