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目的:建立心脑欣胶囊中甜菜碱的含量测定方法。方法:采用LC-MS/MS法测定甜菜碱的含量,以电喷雾离子源(ESI)正离子模式将样品离子化,多反应监测(MRM)模式下对甜菜碱(m/z 118.20→57.92和118.20→59.00)进行测定。色谱柱为Venusil HILIC(100 mm×2.1 mm×3μm),流动相为乙腈-水(80:20),流速为0.3 ml·min~(-1),质谱检测。结果:甜菜碱浓度在4.44~177.6 ng·ml~(-1)范围内线性关系良好(r=0.999 9),平均回收率为99.8%,RSD为1.1%(n=6)。结论:该方法简便、可靠、准确,可作为心脑欣胶囊中甜菜碱的含量测定方法。
Objective: To establish a method for the determination of betaine in Xinnaoxin capsule. Methods: The content of betaine was determined by LC-MS / MS. The sample was ionized by positive ion electrospray ionization (ESI) mode. The effect of betaine (m / z 118.20 → 57.92 and 118.20 → 59.00). The chromatographic column was Venusil HILIC (100 mm × 2.1 mm × 3 μm). The mobile phase was acetonitrile-water (80:20) and the flow rate was 0.3 ml · min -1. Results: The linearity of betaine ranged from 4.44 to 177.6 ng · ml ~ (-1) (r = 0.999 9). The average recovery was 99.8% and the RSD was 1.1% (n = 6). Conclusion: The method is simple, reliable and accurate and can be used as a method for the determination of betaine in Xinnaoxin capsule.