论文部分内容阅读
作者取猪眼球,在显微镜下分离虹膜、睫状体、小梁细胞,部分小梁组织培养。各组织块通过匀浆、离心得到mRNA。培养的小梁组织用磷酸缓冲液冲洗。细胞在avzian myelblastosis病毒逆转录酶作用下合成cDAN,PCR扩增,30bp的寡核苷酸探针行Southein blot。收集小梁细胞培养液,无血清培养8h,3次;再培养24h,3次。离心后用~(125)I-TGFβ_1测定。 结果发现,猪的小梁细胞含有TGF_1β_1 mRNA,能分泌和产生TGFβ_1。在磷酸缓冲液冲洗,无血清反复培养后培养液中测得的TGFβ_1不是血清携带的,而是小梁细胞自身分泌所致。
The author took the pig’s eye ball, the iris, ciliary body, trabecular cells under the microscope, part of trabecular tissue culture. Each tissue mass was homogenized and centrifuged to obtain mRNA. The cultured trabecular tissue is washed with phosphate buffer. The cells were cDAN synthesized by the avzian myelblastosis virus reverse transcriptase, PCR amplification and 30 bp oligonucleotide probe Southein blot. Trabecular meshwork cells were collected, serum-free culture 8h, 3 times; then cultured 24h, 3 times. After centrifugation with ~ (125) I-TGFβ_1 determination. The results showed that pig trabecular cells contain TGF_1β_1 mRNA, can secrete and produce TGFβ_1. In phosphate buffered saline rinse, serum-free repeated culture of TGFβ_1 measured in the culture medium is not carried by the serum, but the trabecular cells themselves secreted.