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目的 :检测强直性脊柱炎(ankylosing spoondylitis,AS)患者血清中抗CD74抗体的水平,并评估抗CD74抗体在AS患者中的诊断价值。方法:选取确诊为AS的85例汉族患者和46名健康献血者(对照),采集血清后用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)法,用2种重组人CD74蛋白和1种CD74肽作为抗原,检测抗CD74抗体水平,并采用独立样本t检验和单向方差检验进行组间均值比较。同时比较不同冻存时间的血标本中抗CD74抗体水平差异。结果:3种抗原检测出的AS患者抗CD74抗体的吸光度值(A)均值分别为0.281±0.189、0.157±0.058和0.120±0.040,与健康对照组相比,差异均无统计学意义;而AS患者中抗CD74抗体的阳性率非常低,且在不同年龄组及不同性别间抗CD74抗体水平差异无统计学意义。结论:采用不同抗原测得的抗CD74抗体水平在85例汉族AS患者中未见显著升高,考虑其在我国汉族人群中其可能并不能作为诊断AS有价值的指标。
Objective: To detect the serum level of anti-CD74 antibody in patients with ankylosing spondylitis (AS) and to evaluate the diagnostic value of anti-CD74 antibody in patients with AS. Methods: A total of 85 Han patients and 46 healthy donors were enrolled in the study. Serum was collected and analyzed by enzyme-linked immunosorbent assay (ELISA) and two recombinant human CD74 proteins and 1 CD74 peptides were used as antigens to detect anti-CD74 antibody levels and the means of group comparisons between groups using the independent sample t-test and the one-way test of variance. At the same time, the differences of anti-CD74 antibody in blood samples with different cryopreservation time were compared. Results: The mean values of absorbance (A) of anti-CD74 antibodies in AS patients detected by three antigens were 0.281 ± 0.189,0.157 ± 0.058 and 0.120 ± 0.040, respectively, which were not significantly different from those in healthy controls The positive rate of anti-CD74 antibody in patients was very low, and there was no significant difference in anti-CD74 antibody level between different age groups and different sexes. Conclusion: The level of anti-CD74 antibody measured by different antigens has not been significantly increased in 85 Han patients with AS. It may not be considered as a valuable index in diagnosing AS in Chinese Han population.