论文部分内容阅读
目的探讨d-δ-三烯生育酚对人鼻咽癌细胞增殖与凋亡的影响。方法实验设对照组和不同d-δ-三烯生育酚浓度(40、50、60μmol/L)处理的实验组,采用Hoechst荧光染料染色和显微镜观察细胞的形态。3-(4,5二甲基噻唑-2)-2,5-二苯基四氮唑溴蓝(MTT)实验、流式细胞术分析d-δ-三烯生育酚对CNE2细胞的增殖、周期和凋亡的影响。结果 d-δ-三烯生育酚处理CNE2细胞48 h后,随d-δ-三烯生育酚的浓度升高,细胞生长抑制率明显增加,分别为(27.71±1.01)%、(57.09±0.56)%和(95.04±0.17)%,与对照组相比,差异具有统计学意义(P<0.01);细胞核或细胞浆内出现致密浓染的颗粒状或条块状荧光的凋亡细胞明显增多,实验组细胞凋亡率分别为(9.18±1.86)%、(34.97±1.01)%和(52.97±2.77)%,与对照组(1.58±0.48)%比较,随浓度升高,凋亡细胞率增加,差异具有统计学意义(P<0.01);实验组S期的细胞百分率分别为(25.40±1.93)%、(35.83±3.74)%和(47.13±2.49)%,与对照组(19.63±1.46)%比较,细胞呈S期阻滞,差异具有统计学意义(P<0.05)。结论 d-δ-三烯生育酚具有抗鼻咽癌CNE2细胞的生物学活性。
Objective To investigate the effects of d-δ-tocotrienol on proliferation and apoptosis of human nasopharyngeal carcinoma cells. Methods The experimental group and the control group and different d-δ-tocotrienol concentrations (40,50,60 μmol / L) treatment group, Hoechst staining and microscopic observation of cell morphology. The cytotoxicity of d-δ-tocotrienol on the proliferation of CNE2 cells was analyzed by MTT assay and 3- (4,5-dimethylthiazol-2) -2,5-diphenyltetrazolium bromide (MTT) Cycle and apoptosis. Results After 48 hours treatment of CNE2 cells with d-δ-tocotrienol, the cell growth inhibition rate was significantly increased with the increase of d-δ-tocotrienol concentration (27.71 ± 1.01%, (57.09 ± 0.56 )% And (95.04 ± 0.17)%, respectively. The difference was statistically significant (P <0.01). The number of apoptotic cells in the nucleus or in the cytoplasm of the cells was increased (9.18 ± 1.86)%, (34.97 ± 1.01)% and (52.97 ± 2.77)%, respectively. Compared with the control group (1.58 ± 0.48)%, the apoptotic rate of the experimental group was higher than that of the control group (P <0.01). The percentage of cells in the experimental group at the S phase were (25.40 ± 1.93)%, (35.83 ± 3.74)% and (47.13 ± 2.49)% respectively, which was significantly higher than that in the control group (19.63 ± 1.46 )%, The cells were S phase arrest, the difference was statistically significant (P <0.05). Conclusion d-δ-tocotrienol has the biological activity against nasopharyngeal carcinoma CNE2 cells.