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目的 探讨聚合酶链反应技术 (PCR)在检测石蜡包埋组织中金黄色葡萄球菌DNA的应用及价值。方法 利用PCR技术 ,对 55例福尔马林固定、石蜡包埋小鼠组织中SA DNA进行了检测。结果 55例石蜡包埋的小鼠组织中均检测到SA DNA ,这与临床病理诊断为金黄色葡萄球菌 (S .aureus)感染相一致。结论 PCR技术可用于检测石蜡包埋组织中的SA DNA片段 ,为鼠S .aureus感染的回顾性分析提供了有效手段
Objective To investigate the value and application of polymerase chain reaction (PCR) in the detection of Staphylococcus aureus DNA in paraffin-embedded tissues. Methods The SA DNA in 55 formalin-fixed and paraffin-embedded mice tissues was detected by PCR. Results SA DNA was detected in all 55 paraffin - embedded mice tissues, which was consistent with the clinicopathological diagnosis of S. aureus infection. Conclusion The PCR technique can be used to detect SA DNA fragments in paraffin-embedded tissues, which provides an effective method for the retrospective analysis of S. aureus infection