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为了解连作茶树根部土壤酸化对微生物生物量及区系的影响,以不同种植年限黄金桂茶树根际土壤为研究对象,分析茶树根际土壤p H值对土壤微生物量、微生物数量和微生物类群的影响以及土壤微生物对土壤酸度响应的敏感性趋势.结果显示,茶树根际土壤的微生物生物量碳、微生物呼吸强度、微生物生物量氮及细菌、放线菌、氨化细菌、亚硝酸细菌、反硝化细菌、好气性自生固氮菌、好气性纤维素分解菌的数量随着茶树树龄的增加呈现下降趋势,且与茶树根际土壤p H值呈现极显著正相关(P<0.01),而真菌、嫌气性纤维素分解菌、硫化细菌的数量呈现上升趋势,且与茶树根际土壤p H值呈现极显著正相关(P<0.01).敏感性分析结果显示,不同微生物指标响应茶树根际土壤p H值变化的趋势为微生物呼吸强度(8.721 8)>细菌(8.428 7)>微生物量碳(7.955 2)>硫化细菌(7.726 8)>放线菌(5.780 0)>真菌(4.740 8)>嫌气性纤维素分解菌(4.065 0)>氨化细菌(3.760 6)>好气性自生固氮菌(2.368 3)>反硝化细菌(2.340 6)>微生物量氮(2.324 2)>亚硝酸细菌(2.219 8)>好气性纤维素分解菌(1.772 0).综上表明,茶树根际土壤酸化显著影响了土壤微生物数量变化,不同的微生物指标响应土壤酸度的敏感性存在一定差异.(图3表3参32)
In order to understand the effect of soil acidification on the biomass and flora of tea plantation, the rhizosphere soil of rhizosphere of tea plantation was analyzed for its effect on soil microbial biomass, microbial population and microbial population The results showed that microbial biomass C, microbial respiration, microbial biomass nitrogen and bacteria, actinomycetes, ammoniated bacteria, nitrite bacteria and rhizosphere soil in tea rhizosphere soil The numbers of nitrifying bacteria, aerobic Azotobacter and aerobic cellulolytic bacteria showed a downward trend with the increase of the tea tree age, and showed a significant positive correlation with the p H value in the rhizosphere soil of the tea plant (P <0.01) The number of anaerobic cellulolytic bacteria and sulphurized bacteria showed an upward trend, and showed a significant positive correlation with the p H value in rhizosphere soil of tea plant (P <0.01) .The results of sensitivity analysis showed that different microorganisms responded to the rhizosphere soil The trend of the change in p H value was that of respiration of microorganisms (8.721 8)> bacteria (8.428 7)> microbial biomass carbon (7.955 2)> sulfide bacteria (7.726 8)> actinomycetes (0)> fungi (4.740 8)> anaerobic cellulolytic bacteria (4.065 0)> ammonified bacteria (3.760 6)> aerobic Azotobacter (2.368 3)> denitrifying bacteria (2.340 6)> microbial biomass Nitrogen (2.324 2)> nitrite bacteria (2.219 8)> aerobic cellulolytic bacteria (1.772 0) .To sum up, acidification of tea rhizosphere soil significantly affected the change of soil microbial quantity, and different microbial indicators responded to soil acidity There are some differences in sensitivity (Figure 3 Table 3 Reference 32)