论文部分内容阅读
选择日本鉴别品种新 2号和K5 9,对源于云南省的水稻稻瘟病菌可育性菌株CHL12 4(MAT1 1)和CHL12 5 (MAT1 2 )及其杂交后代 72个子囊菌株的非致病性 (无毒性 )进行了遗传分析。结果表明 ,菌株CHL12 4对新 2号 (含有效抗性基因Pish)和K5 9(Pit)的非致病性分别由非致病性基因Avr2 Pish和Avr2 Pit控制。通过对这 2个基因控制的非致病性反应进行列联卡方测验 ,显示这 2个基因连锁在一起 ,重组率为 2 6 .8%。进一步利用分离群体分析法 ,对这 2个基因进行了RAPD (随机扩增多态性DNA)分析。结果发现 ,2个RAPD标记OPW 0 6 64 5和OPW 1364 5与非致病性基因Avr2 Pish连锁 ,它们的重组率分别为 2 7.8%和 2 6 .5 %;另 1个RAPD标记OPR11517则与非致病性基因Avr2 Pit连锁 ,重组率为 2 3.5 %。
In this study, we selected non-pathogenic strains Nipponbare 2 and K5 9 from japonica rice blast fungus strains CHL12 4 (MAT1 1) and CHL12 5 (MAT1 2) and their hybrid progenies Sexual (non-toxic) Genetic analysis was performed. The results showed that the non-pathogenicity of strain CHL12 4 toxin 2 (containing Pish and P5) (Pit) was controlled by non-pathogenic genes Avr2 Pish and Avr2 Pit, respectively. By the cascade chi-square test on the non-pathogenic reactions controlled by these two genes, the two genes were linked together and the recombination rate was 26.8%. Further analysis of these two genes by RAPD (Random Amplified Polymorphic DNA) was carried out by using segregation population analysis. The results showed that two RAPD markers, OPW 0 6 64 5 and OPW 1364 5, were linked to Avr2 Pish, and their recombination rates were 2 7.8% and 26.5% respectively. Another RAPD marker, OPR11517, Non-pathogenic gene Avr2 Pit chain, the recombination rate of 23.5%.