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本文作者用猪胰脏的α-淀粉酶处理不液化精液后进行体外受精。将病人分为二组:Ⅰ组为115例精液属正常液化的病人,均按标准体外受精方法进行。Ⅱ组为26例45分钟内精液未液化的病人,精液中加入300μl/含1,800单位的α-淀粉酶,并在室温下孵育10分钟,将精液和加入淀粉酶的精液用Percoll不连续的(40和90%)梯度离心(750kg,15分钟)洗涤二次,取90%那层浓度Percoll中的精子使用,如果有缺损和死精子,可不进行第二次洗涤而用精子泳上的步骤代替,取上层液中的精子,在室温下置5%的空气中。卵母细胞和胚胎的培养及受
In this study, we treated in vitro fertilization with liquefied semen from porcine pancreatic α-amylase. The patients were divided into two groups: Group Ⅰ, 115 cases of normal semen liquefaction patients, according to standard in vitro fertilization method. In group II, 26 patients who did not liquefy semen within 45 minutes were semen added with 300 μl / 1,800 units of α-amylase and incubated at room temperature for 10 minutes. Semen and amylase-added semen were treated with discontinuous 40, and 90%) gradient centrifugation (750 kg, 15 minutes) and sperm taken at a concentration of 90% of that in Percoll. If there are defects and dead spermatozoa, a second wash may be performed instead of the sperm wash Take the sperm in the supernatant and set it to 5% air at room temperature. Oocyte and embryo culture and affected