论文部分内容阅读
目的 探讨人膀胱癌中Ha rasmRNA及端粒酶的表达与肿瘤临床指标的关系。方法采用原位杂交及银染端粒重复序扩增程序 (TRAP)反应的方法对 91例膀胱癌标本进行Ha rasmRNA及端粒酶活性的表达检测。结果 91例膀胱癌标本中Ha rasmRNA与端粒酶活性检出率为 73 %与 92 % ,两者阳性率及阳性强度与肿瘤的恶性程度呈正相关 ,在不同临床分期中两者表达差异无显著性 (P >0 .0 5 )。结论 Ha ras癌基因与端粒酶是参与肿瘤的发生并影响肿瘤发展的重要因素 ,有望成为判断肿瘤恶性程度、自然病程及预后的评估指标。
Objective To investigate the relationship between the expression of Ha rasmRNA and telomerase in human bladder cancer and its clinical significance. Methods The expression of Ha rasmRNA and telomerase activity in 91 cases of bladder cancer specimens were detected by in situ hybridization and TRAP (silver-labeled telomere repeat amplification program). Results The positive rates of Ha rasmRNA and telomerase in 91 cases of bladder cancer were 73% and 92%, respectively. The positive rate and the positive rate of Ha rasmRNA were positively correlated with the malignant degree of the tumor. There was no significant difference in the expression of Ha rasmRNA and telomerase between the two groups Sex (P> 0.05). Conclusion Ha ras oncogene and telomerase are important factors involved in the occurrence of tumor and affect tumor development. It is expected to be an evaluation index to determine the degree of malignancy, natural course of disease and prognosis.