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背景:饥饿是脂肪代谢的一个重要过程,可触发适应性反应,瘦素水平下降,但饥饿时脑中瘦素受体是否变化及变化情况尚不清楚。目的:饥饿状态下瘦素受体表达的变化及杏仁基底外侧核和中央核的作用。设计:随机对照的实验研究。地点和材料:实验地点:西安交通大学医学院。成年健康雄性SD大鼠20只,随机分为禁食24,48,72h组和对照组,每组5只。干预措施:各组大鼠禁食前后分别检测体质量,体长,尾长。采用放免法测定血清瘦素水平,免疫组化ABC法检测瘦素受体和神经细丝酸性蛋白(glialfibrillaryacidicprotein,GFAP)的表达情况。主要观察指标:①各组大鼠体质量减少量,Lee指数,血清瘦素水平和血糖水平。②各组大鼠瘦素受体的表达。③各组大鼠星形胶质细胞GFAP的表达。结果:禁食24h血清瘦素由(2.49±0.60)μg/L下降至(1.26±0.97)μg/L,体质量减轻4.22%,48h和72h体质量分别减轻12.15%和19.70%,血清瘦素分别降至(0.78±0.19)μg/L和(0.62±0.16)μg/L。与正常大鼠相比,禁食24h杏仁基底外侧核瘦素受体表达增强犤LR-IR细胞数(131±8.9)个/mm2,(68±10.4)个/mm2犦,禁食48h瘦素受体表达水平(82±12.6)个/mm2接近正常大鼠,禁食72h瘦素受体阳性细胞明显减少(49.0±7.4)个/mm2。杏仁中央核瘦素受体表达在禁食前后差异无显著性意义
BACKGROUND: Starvation is an important process of fat metabolism that triggers adaptive responses and decreases leptin levels. However, it is unclear whether the leptin receptor changes in the brain during starvation. OBJECTIVE: To investigate the changes of leptin receptor expression in starvation and the role of basolateral and central almond nuclei. Design: Randomized controlled experimental study. Venue and Materials: Experimental Location: Xi’an Jiao Tong University School of Medicine. Twenty healthy adult male Sprague-Dawley rats were randomly divided into fasting 24,48,72h group and control group, with 5 rats in each group. Interventions: The body weight, body length and tail length of the rats in each group before and after fasting. Serum leptin levels were measured by radioimmunoassay and immunohistochemistry ABC method was used to detect the expression of leptin receptor and glial fibrillary acidic protein (glialfibrillary acid protein, GFAP). MAIN OUTCOME MEASURES: ① The body weight loss, Lee index, serum leptin level and blood glucose level of rats in each group. ② The expression of leptin receptor in each group. ③ The expression of GFAP in astrocytes of rats in each group. Results: After fasting for 24 h, serum leptin decreased from (2.49 ± 0.60) μg / L to (1.26 ± 0.97) μg / L, body weight decreased by 4.22%, body weight decreased by 12.15% and 19.70% at 48 and 72 hours, (0.78 ± 0.19) μg / L and (0.62 ± 0.16) μg / L, respectively. Compared with normal rats, the expression of leptin receptor in the basolateral basal fastidious cells was increased after fasting for 24 h (131 ± 8.9) / mm2, (68 ± 10.4) / mm2, and the fasting 48h leptin The receptor expression level (82 ± 12.6) / mm2 was close to that of normal rats, and the number of leptin receptor positive cells decreased significantly (49.0 ± 7.4) / mm2 72h after fasting. There was no significant difference in the expression of central amnion receptor in almond before and after fasting