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神经脱髓鞘疾病是中枢神经系统难治性疾病,髓鞘脱失后伴有各种基因表达的异常。明确这些基因的表达形式对脱髓鞘疾病的治疗具有重要意义。本研究利用0.2%cuprizone饲育C57BL/6小鼠6周制备中枢神经脱髓鞘模型,采用原位杂交技术对组织蛋白酶抑制剂cystatin F的mRNA表达进行研究。结果发现,正常野生型小鼠中枢神经系统中未见cystatin F mRNA的表达,而大量表达于脱髓鞘模型小鼠白质内。通过小胶质细胞标志物Iba1抗体免疫组化和c-fms的原位杂交研究发现,脱髓鞘模型小鼠白质内有大量激活的小胶质细胞。为进一步确定表达cystatin F的细胞类型,通过cystatin F原位杂交后Iba1免疫双染技术,确定cystatin F在中枢神经主要由激活的小胶质细胞表达,提示其表达与小胶质细胞活化和崩解髓鞘残骸清除有关。
Nerve demyelinating disease is a refractory disease of the central nervous system. After demyelination, there are abnormalities of various gene expressions. Clear expression of these genes on the demyelinating disease treatment is of great significance. In this study, C57BL / 6 mice were fed with 0.2% cuprizone for 6 weeks to prepare central nervous demyelination model, and the expression of cystatin F mRNA was studied by in situ hybridization. The results showed that cystatin F mRNA expression was not found in the central nervous system of normal wild-type mice, but was abundantly expressed in the white matter of demyelinated mice. By microglial marker Iba1 antibody immunohistochemistry and c-fms in situ hybridization study found that a large number of demyelinated mouse white matter activated microglial cells. To further determine the cystatin F-expressing cell types, cystatin F was identified mainly by activated microglia in the central nervous system by double staining of Iba1 after cystatin F in situ hybridization, suggesting that the expression of cystatin F is associated with microglial activation and collapse Demyelinating debris removal related.