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[目的]建立香砂养胃丸(浓缩丸)中厚朴的厚朴酚、和厚朴酚含量的测定方法。[方法]采用高效液相法,色谱柱:Diamonsil C18(250 mm×4.6 mm,5μm);流动相:乙腈?水?冰醋酸(55∶43∶2);检测波长为294 nm;流速为1.0 ml/min;进样量为20μl。[结果]厚朴酚在0.190 2～3.804 0μg,和厚朴酚在0.092 222～1.844 400μg时与峰面积呈良好的线性关系,厚朴酚、和厚朴酚平均回收率97.0%,RSD为1.7%。[结论]该方法准确、操作方便,可用于该产品的质量控制鉴定,为建立完整质量标准提供了依据。
[Objective] To establish a method for the determination of magnolol and honokiol in Xiangsha Yangwei Pills (enriched pills). [Methods] High-performance liquid chromatography was used. The chromatographic column was Diamonsil C18 (250 mm×4.6 mm, 5 μm). The mobile phase consisted of acetonitrile, water and glacial acetic acid (55∶43∶2). The detection wavelength was 294 nm. The flow rate was 1.0. Ml/min; injection volume is 20 μl. [Results] The results showed that the honokiol ranged from 0.190 2 to 3.804 0 μg and honokiol from 0.092 222 to 1.844 400 μg showed a good linear relationship with the peak area. The average recovery of honokiol and honokiol was 97.0%, and the RSD was 1.7. %. [Conclusion] The method is accurate and easy to operate. It can be used for the quality control and appraisal of the product and provides a basis for establishing a complete quality standard.