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目的探讨云芝氯仿萃取物诱导人肝癌HepG-2细胞凋亡作用。方法常规培养人肝癌HepG-2细胞,分为对照组、云芝氯仿萃取物处理组(终浓度分别为100,200,400 mg/L),通过HE染色、PCR和Western Blotting方法检测云芝氯仿萃取物对人肝癌HepG-2细胞组织形态学变化、DNA降解片段、Bc1-2,Bax和Caspase-3蛋白表达变化。结果100,200mg/L和400 mg/L云芝氯仿萃取物作用48 h后,人肝癌HepG-2细胞DNA降解成DNA片段,且随着剂量增加,降解作用越明显;随剂量增加Bc1-2/Bax值逐渐下降、Caspase-3蛋白表达显著升高。结论云芝氯仿萃取物可以诱导人肝癌HepG-2细胞凋亡。
Objective To investigate the apoptosis of HepG-2 cells induced by chloroform-chloroform extract. Methods Human hepatocellular carcinoma HepG-2 cells were routinely cultured and divided into control group and chlorophyll-chloroform extract group (final concentrations were 100, 200, and 400 mg / L, respectively). HE staining, PCR and Western Blotting were used to detect the effects of chloroform extract Histological changes, DNA degradation fragments, Bc1-2, Bax and Caspase-3 protein expression in HepG-2 cells. Results The DNA of human hepatocellular carcinoma HepG-2 cells degraded into DNA fragments after 100, 200 mg / L and 400 mg / L chlorophyll a extracts for 48 h. With the increase of dose, the degradation was more obvious. With the increase of dose of Bc1-2 / Bax value gradually decreased, Caspase-3 protein expression increased significantly. Conclusion Chlorophyll fluorescence extract can induce apoptosis of HepG-2 cells.