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为了明确植物源活性成分芪类化合物在农业病害防治中的前景,在离体条件下分别测定了白藜芦醇(Ⅰ)及其衍生物(Ⅱ～Ⅴ)对植物病原真菌菌丝生长的抑制作用以及对菌丝形态和孢子萌发的影响,采用叶片法和温室盆栽法研究了白藜芦醇的防病作用原理。结果表明:5个供试化合物对6种供试病原菌的菌丝生长均有不同程度的抑制作用,但均对番茄早疫病菌Alternaria solani的抑制活性最高,其中又以3,5-二羟基-4’-甲氧基二苯乙烯(Ⅲ)的抑制活性最高;白藜芦醇可造成番茄早疫病菌菌丝体畸形,并可抑制该病菌孢子的萌发,但未引起孢子形态改变。叶片法和温室盆栽法试验的结论一致,即白藜芦醇能够抑制病原菌的侵染,对植株起到一定的保护作用。
In order to clarify the prospect of the plant-derived active ingredient stilbene compounds in the prevention and control of agricultural diseases, the inhibition of the mycelial growth of phytopathogenic fungi was determined in vitro under the conditions of resveratrol (Ⅰ) and its derivatives (Ⅱ ~ Ⅴ) Role and the impact on the morphology of mycelium and spore germination, leaf method and greenhouse pot method to study the role of resveratrol in disease prevention. The results showed that all of the five tested compounds inhibited the mycelial growth of six tested pathogens to varying degrees, but all of them had the highest inhibitory activity against Alternaria solani against tomato Alternaria solani. Among them, 3,5 - dihydroxy - 4’-methoxystilbene (Ⅲ) had the highest inhibitory activity. Resveratrol could cause the mycelia deformity of A. solani and inhibited the spore germination but did not cause the spore morphological changes. Leaf method and greenhouse pot experiment concluded that resveratrol can inhibit the infection of pathogenic bacteria play a protective effect on plants.