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本文利用糠醛-硫酸比色法测定速效伤风胶囊中人工牛黄(Ⅰ)的胆酸含量。取10粒胶囊研细,将样粉(约含Ⅰ2mg)用氯仿提取。过滤,在80℃水浴上蒸去溶剂,冷却,加60%醋酸溶液溶解Ⅰ并稀释至25ml。将此溶液2.0ml及1%糠醛溶液(Ⅱ)1.0ml混合,冰浴上冷却5min,加入硫酸溶液并混匀。用分光光度法于605nm以空白对照测量吸收值。每粒中约含Ⅰ为5,10,15mg时回收率分别为93.56,94.39及97.62%;变异系数分别为0.13(n=3),1.2(n=6)及0.26%(n=3)。
Furfural - sulfuric acid colorimetry was used to determine the bile acid content of Artificial Bezoar (Ⅰ) Take 10 capsules research fine, the sample powder (about I2mg) extracted with chloroform. Filtration, the solvent was evaporated at 80 ℃ water bath, cooled, dissolved in 60% acetic acid solution Ⅰ and diluted to 25ml. 2.0ml of this solution and 1.0ml of 1% furfural solution (II) were mixed, cooled on ice bath for 5min, added with sulfuric acid solution and mixed well. Absorbance was measured by spectrophotometry at 605 nm with a blank control. The recoveries were about 93.56, 94.39 and 97.62% when the content of I was 5,10 and 15mg in each tablet. The coefficient of variation was 0.13 (n = 3), 1.2 (n = 6) and 0.26% (n = 3) respectively.