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目的对我国广西登革3型病毒分离株桂登-1株(GD-1株)和桂登-11株(GD-11株)进行全基因组序列测定和分析,为了解其地理来源提供依据。方法根据GenBank提交的登革3型病毒基因序列设计9对引物,RT-PCR方法分段扩增GD-1和GD-11株基因序列,测序后进行拼接,得到其全基因组序列。结果两株病毒全长均为10 707nt,与国际参考株H87株核苷酸同源性为96.0%,氨基酸同源性为98.8%。GD-1株和GD-11株间仅有4个氨基酸差异,二者与H87株分别存在39和41个氨基酸差异。对3’UTR二级结构进行预测,二者与H87株存在较大差异。根据E蛋白基因序列对两株病毒进行进化分析,GD-1和GD-11均属于亚型Ⅱ,与分离自泰国的两毒株进化关系较近。结论 GD-1和GD-11均属于登革3型病毒亚型Ⅱ,二者可能是源自泰国的病原。
Objective To investigate the genome-wide sequence analysis and sequence analysis of Guandong-1 strain (GD-1 strain) and Guidong-11 strain (GD-11 strain) of Dengue virus type 3 in Guangxi and provide the basis for understanding its geographical origin. Methods Nine pairs of primers were designed according to the sequence of dengue virus type 3 (GenBank). The sequences of GD-1 and GD-11 were amplified by RT-PCR and sequenced. The whole genome sequence was obtained. Results The total length of the two viruses was 10 707 nt. The homology was 96.0% with the international reference strain H87 and the amino acid homology was 98.8%. There were only 4 amino acid differences between GD-1 and GD-11 strains, and there were 39 and 41 amino acid differences between the two strains and H87 strain respectively. The secondary structure of 3’UTR was predicted. There was a big difference between the two and H87 strain. Based on the E protein gene sequence analysis of two viruses, both GD-1 and GD-11 belonged to subtype Ⅱ, which evolved closer to the two strains isolated from Thailand. Conclusion Both GD-1 and GD-11 belong to Dengue 3 virus subtype Ⅱ, both of which may originate from Thailand.