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在体外观察全反式维甲酸对人肺腺癌细胞株SPC-A1的增殖和DNA、RNA以及蛋白质合成的影响。当细胞在含有5μmol/L、10μmol／L和20μmol／L维甲酸的培养液中培养时，发现细胞生长均受到明显抑制。细胞在含有10μmol／L维甲酸的培养液中培养2天后，其3H—TdR和3H-亮氨酸掺入率明显下降，而培养延长至4天后，3H—TdR、3H—UdR和3H-亮氨酸掺入率都明显下降。通过流式细胞仪分析发现经过维甲酸处理6天后的细胞，其G1／G1期的细胞（67．1％）明显高于对照组（52．1％）（P＜0．01），而S期细胞（19．6％）明显低于对照组（35．8％）（P＜001）。
The effect of all-trans retinoic acid on proliferation and DNA, RNA and protein synthesis of human lung adenocarcinoma cell line SPC-A1 was observed in vitro. When cells were cultured in a medium containing 5 μmol/L, 10 μmol/L, and 20 μmol/L retinoids, cell growth was found to be significantly inhibited. After the cells were cultured in a medium containing 10 μmol/L retinoid for 2 days, the incorporation rate of 3H-TdR and 3H-leucine was significantly decreased. After the culture was extended to 4 days, the 3H-TdR, 3H-UdR, and 3H- were bright. The rate of incorporation of arginine decreased significantly. After flow cytometry analysis, cells in the G1/G1 phase (67.1%) were significantly higher than those in the control group (52.1%) after 6 days of retinoic acid treatment (P<0.01). The period cells (19.6%) were significantly lower than the control group (35.8%) (P < 001).