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目的:检测大肠癌组织中A3AR蛋白表达和A3AR mRNA基因扩增,并探讨其与临床病理特征的关系。方法:分别用免疫组化和实时荧光定量PCR方法检测31例大肠癌组织和10例癌旁正常组织中A3AR蛋白表达及其A3AR mRNA扩增。结果:大肠癌及癌旁正常组织中A3AR的阳性率分别为61.29%(19/31)和10.00%(1/10),差异有统计学意义,P<0.05。大肠癌及癌旁正常组织中A3AR mR-NA扩增分别是0.034 8±0.019 5和0.021 4±0.014 5,两组差异有统计学意义,P<0.05。两组的表达均与大肠癌的临床分期和淋巴结转移密切相关。结论:大肠癌组织A3AR在蛋白和基因水平均升高,与临床分期和淋巴结转移相关,提示可能参与了大肠癌的侵袭和转移。
OBJECTIVE: To detect the expression of A3AR protein and A3AR mRNA in colorectal carcinoma and to explore its relationship with clinical pathological features. Methods: The expression of A3AR protein and its A3AR mRNA in 31 cases of colorectal carcinoma and 10 cases of adjacent normal tissues were detected by immunohistochemistry and real-time fluorescence quantitative PCR respectively. Results: The positive rates of A3AR in colorectal cancer and adjacent normal tissues were 61.29% (19/31) and 10.00% (1/10), respectively, with statistical significance (P <0.05). The amplification of A3AR mR-NA in colorectal cancer and adjacent normal tissues was 0.034 8 ± 0.019 5 and 0.021 4 ± 0.014 5, respectively, with significant difference between the two groups (P <0.05). The expression of both groups was closely related to the clinical stage and lymph node metastasis of colorectal cancer. Conclusion: The expression of A3AR protein in colorectal cancer is higher than that in clinical stage and lymph node metastasis, suggesting that it may be involved in the invasion and metastasis of colorectal cancer.