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目的:探讨姜黄素对免疫性前列腺炎大鼠前列腺组织肿瘤坏死因子-α(tumor necrosis factor alpha,TNF-α)、核因子-κB(nuclear factorκB,NF-κB)、环氧合酶-2(cyclooxygenase-2,COX-2)表达的影响。方法:将60只SD雄性大鼠随机分为4组:正常对照组、模型组、姜黄素腹腔注射组及塞来昔布组(阳性对照组),每组15只。大鼠腹腔注射百白破疫苗同时皮下多点注射经过乳化的前列腺蛋白提纯液和免疫佐剂构建免疫性前列腺炎模型。造模成功后,连续给药四周。酶联免疫吸附法(enzyme linked immunosorbent assay,ELISA)检测前列腺组织中TNF-α的表达,免疫组织化学法测定前列腺组织NF-κB(p65)水平变化,免疫荧光测定前列腺组织COX-2水平变化,Western blot法测定前列腺组织NF-κB(p65)及COX-2的表达。结果:模型组大鼠前列腺组织中TNF-α(F=382.730,P=0.000)、NF-κB(p65)(F=56.230,P=0.000)、COX-2(F=122.290,P=0.000)的表达明显高于其他3组。姜黄素治疗组大鼠前列腺组织中TNF-α、COX-2表达明显低于塞来昔布治疗组(P<0.01),而NF-κB(p65)的表达无明显差异(P=0.059)。结论:姜黄素对免疫性前列腺炎大鼠有一定的治疗作用,其机制可能与降低前列腺组织中炎性细胞因子TNF-α、NF-κB(p65)、COX-2水平有关。
Objective: To investigate the effect of curcumin on the expression of tumor necrosis factor alpha (TNF-α), nuclear factor κB (NF-κB), cyclooxygenase-2 cyclooxygenase-2, COX-2) expression. Methods: Sixty SD male rats were randomly divided into 4 groups: normal control group, model group, curcumin intraperitoneal injection group and celecoxib group (positive control group), 15 rats in each group. Rats were injected intraperitoneally with diphtheria-baicalein vaccine and subcutaneously injected with emulsified prostatic protein purification solution and immune adjuvant to construct an immune prostatitis model. After successful modeling, continuous administration for four weeks. The expression of TNF-α in prostate tissue was detected by enzyme-linked immunosorbent assay (ELISA), the level of NF-κB (p65) in prostatic tissue was detected by immunohistochemistry, the level of COX-2 in prostatic tissue was detected by immunofluorescence, Western blot was used to detect the expression of NF-κB (p65) and COX-2 in prostate tissue. Results: The levels of TNF-α (F = 382.730, P = 0.000), NF-κB p65 (F = 56.230, P = 0.000) and COX-2 The expression was significantly higher than the other three groups. The expression of TNF-α and COX-2 in curcumin-treated rats was significantly lower than that in celecoxib-treated rats (P <0.01), while the expression of NF-κB (p65) was not significantly different (P = 0.059). CONCLUSION: Curcumin has a certain therapeutic effect on immune prostatitis in rats. The mechanism may be related to decreasing the levels of inflammatory cytokines TNF-α, NF-κB (p65) and COX-2 in prostate tissue.