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目的 为探讨用腺病毒载体携带野生型P53基因作为前列腺癌基因治疗的可能性。方法 应用重组人携带野生型P53基因病毒 (Ad WTP53)感染培养的前列腺癌细胞 ,观察表达P53基因蛋白的癌细胞与对照组癌细胞的体外生长和裸鼠体内致瘤能力变化 ,对荷瘤裸鼠瘤体周围注射Ad WTP53,观察治疗组和对照组肿瘤生长的变化。结果 感染Ad WTP53的前列腺细胞体外生长和裸鼠体内致瘤能力明显下降 ,荷瘤裸鼠瘤体周围注射Ad WTP53后肿瘤生长速度明显减慢。结论 证实了野生型P53基因是一种抑癌基因 ,提示其可能被应用于前列腺癌的基因治疗研究
Objective To investigate the possibility of adenovirus vector carrying wild-type P53 gene as a gene therapy for prostate cancer. Methods Human prostate cancer cells were infected with wild type P53 virus (Ad WTP53). The growth of cancer cells in vitro and in vivo were observed in vivo. The in vivo tumorigenicity of P53 gene - The mice were injected with Ad WTP53 around the tumor to observe the changes of the tumor growth in the treatment group and the control group. Results The growth of prostatic cells infected with Ad WTP53 in vitro and tumorigenicity in nude mice were significantly decreased. The tumor growth rate was significantly decreased after Ad WTP53 injection into tumor-bearing nude mice. The results confirmed that the wild-type P53 gene is a tumor suppressor gene, suggesting that it may be applied to the study of gene therapy of prostate cancer