论文部分内容阅读
建立了一种测定葡萄籽提取物中原花青素平均聚合度的新方法。该方法首先以甲醇为溶剂,以传统香草醛法测定原花青素的质量,再以乙酸为溶剂,使香草醛只与原花青素末端黄烷-3-醇反应来测定原花青素物质的量,然后通过对比获得原花青素的平均聚合度。优化后测定原花青素样品物质的量的线性浓度范围为0.028～0.345μmol/mL,检测限为2.97×10-4μmol/mL,加标回收率为95.2%～99.6%,精密度(RSD)为0.78%～0.98%。通过该法测定的样品平均聚合度与实际值接近,具有简单、快速、准确、灵敏等优点。
A new method for the determination of the average degree of polymerization of proanthocyanidins in grape seed extract was established. The method first uses methanol as a solvent to determine the quality of the proanthocyanidin by the traditional vanillin method, and then uses the acetic acid as the solvent to make the vanillin react only with the proanthocyanidin end-flavan-3-ol to measure the amount of the proanthocyanidin material, and then obtains the proanthocyanidin Average degree of polymerization. The optimum concentration of procyanidins was 0.028 ~ 0.345μmol / mL, the detection limit was 2.97 × 10-4μmol / mL, the spiked recoveries were 95.2% ~ 99.6% and the precision was 0.78% ~ 0.98%. The average polymerization degree of the sample measured by the method is close to the actual value, which is simple, fast, accurate and sensitive.