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以菊芋幼茎、块茎为试验材料,比较不同激素浓度配比对外植体培养的影响研究.结果表明:幼茎是试验的最佳外植体,愈伤组织诱导快,且有腋芽发生;不定芽分化适宜培养基是MS+BA 2.0mg/L+NAA 0.01mg/L,不定芽分化率100%;在增殖培养基中不定芽增殖系数达3.25,切分后的茎段分化腋芽或不定芽的增殖系数平均为9.6;不定芽在MS+NAA 0.01mg/L培养基上生根率为93%,试管苗经炼苗后移栽成活率达到91.5%.
The results showed that young shoots were the best explants for the experiment, callus induction was fast, and axillary buds occurred; The optimum medium for bud differentiation was MS + BA 2.0 mg / L + NAA 0.01 mg / L, and the rate of adventitious bud differentiation was 100%. The multiplication coefficient of adventitious buds reached 3.25 in multiplication medium. The differentiated axillary buds or adventitious buds The average multiplication coefficient was 9.6. The rooting rate of adventitious buds on MS + NAA 0.01 mg / L medium was 93%, and the survival rate of transplanting test tube seedlings reached 91.5%.