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为了探讨慢性饮水染镉(Cd)对大鼠血液、睾丸中某些酶活性的影响,试验将30只近交系SD大鼠(雄性,170 g左右)随机分成5组,即对照组、试验1组、试验2组、试验3组、试验4组,分别饮用Cd(Cd Cl2)质量浓度为0,25,50,100,200 mg/L的饮水。56 d后宰杀大鼠,测定血液中和睾丸中镉的蓄积量及碱性磷酸酶(ALP)、乳酸脱氢酶同工酶-X(LDH-X)、酸性磷酸酶(ACP)、乳酸脱氢酶(LDH)、葡萄糖-6-磷酸脱氢酶(G-6-PD)、山梨醇脱氢酶(SDH)、天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、γ-L-谷氨酰转肽酶(GGT)活性。结果表明:与对照组相比,试验1组大鼠血浆LDH活性降低;血浆AST和ALT活性的剂量效应不规律,试验2组有降低现象,而且均只有试验4组显著升高(P<0.05)。与对照组相比,试验2组睾丸中ALP活性显著降低(P<0.05);试验3组中,除LDH-X和ACP外,其他酶活性均显著降低(P<0.05);试验4组各酶活性均显著降低(P<0.05)。慢性饮水染镉,睾丸中某些酶活性的改变较血液中敏感,睾丸中LDH和ALP可以作为反映慢性饮水染镉雄性生殖毒性较敏感的生化指标。
In order to investigate the effect of chronic drinking water cadmium (Cd) on the activity of some enzymes in blood and testis of rats, 30 inbred SD rats (male, about 170 g) were randomly divided into 5 groups, ie control group, One group, two experimental groups, three experimental groups and four experimental groups were drank drinking water with CdCl2 concentrations of 0, 25, 50, 100 and 200 mg / L, respectively. Fifty-six days later, the rats were sacrificed and the amount of cadmium accumulated in the blood and testis was measured. The activities of alkaline phosphatase (ALP), lactate dehydrogenase isoenzyme-X (LDH-X), acid phosphatase (ACP) (LDH, G-6-PD), sorbitol dehydrogenase (SDH), aspartate aminotransferase (AST), alanine aminotransferase ), Γ-L-glutamyl transpeptidase (GGT) activity. The results showed that compared with the control group, the LDH activity of plasma in experimental group 1 was decreased. The dose effect of plasma AST and ALT activity was irregular. The decrease of plasma LDH was observed in both experimental groups, and only in experimental group 4 (P <0.05 ). Compared with the control group, the activity of ALP in Testis 2 group was significantly decreased (P <0.05). In the other three groups, except for LDH-X and ACP, the activities of other enzymes were significantly decreased (P <0.05) Enzyme activity was significantly reduced (P <0.05). Chronic drinking of cadmium, testicular changes in some of the enzyme activity than the blood-sensitive testicular LDH and ALP can be used as a reflection of chronic drinking water cadmium male reproductive toxicity more sensitive biochemical indicators.