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目的探索放线菌酮和ＴＮＦ。协同诱导人大肠癌Ｌ。ＶＯ细胞凋亡的发生规律。方法用细胞体外培养方法，以放线菌酮和均小剂量作用于人大肠癌ＬｏＶｏ细胞，经Ｈｏｅｃｈｓｔ３３２５８荧光染色，光镜观察形态改变，并用流式细胞术检测ＤＮＡ断裂情况。结果单用ＴＮＦａ５万［Ｊ／Ｌ或放线菌酮４ｍｇ／Ｌ，均未引起ＬｏＶｏ细胞凋亡效应。而两者协同，作用１２ｈ至４８ｈ，ＬＯＶＯ细胞逐渐出现明显的凋亡形态学改变和ＤＮＡ断裂。结论小剂量的放线菌阴和ＴＮＦＱ协同作用于人大肠癌Ｌ。ＶＯ细胞，出现明显的凋亡效应。
Objective To explore cycloheximide and TNF. Synergistic induction of colorectal cancer. Occurrence of apoptosis in VO cells. Methods The cells were cultured in vitro with actinomycinone and at low doses. The cells were stained with Hoechst 33258, and the morphological changes were observed by light microscopy. DNA fragmentation was detected by flow cytometry. Results The single use of TNFa50 000 [J/L or cycloheximide 4 mg/L, did not induce apoptosis in LoVo cells. The synergistic effect of the two, the effect of 12h to 48h, LOVO cells gradually appeared apoptotic morphological changes and DNA fragmentation. Conclusion Low-dose actinomyces Yin and TNFQ act synergistically on human colorectal cancer L. In VO cells, there was a significant apoptotic effect.