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目的：寻找人肝细胞癌（ＨＣＣ）特异抗原。方法：应用重组克隆表达抗原的血清学鉴定技术（ＳＥＲＥＸ），用病人自身血清从广西肝细胞癌ｃＤＮＡ文库中筛选出编码ＨＣＣ肿瘤抗原的基因克隆，用肝癌患者及其他人血清检验ＨＣＣ肿瘤抗原对肝癌的特异性，测定抗原ＤＮＡ序列并与基因库核对寻找其同源结构。结果：其中一种ＨＣＣ肿瘤抗原与衰老标记蛋白－３０（ＳＭＰ－３０）是同源结构。相应抗体主要只见于ＨＣＣ病人，在２０例其它５种恶性肿瘤和４例肝硬化病人未查到相应抗体，２０例慢性肝炎病人血清和２０例正常人血清均只见１例呈阳性反应。结论：衰老标记蛋白－３０为一种新发现的ＨＣＣ相关抗原，对ＨＣＣ有诊断学意义。
Objective: To search for human hepatocellular carcinoma (HCC) specific antigen. Methods: Using the serological identification technique (SEREX) for the expression of recombinant clones to express antigens, a gene clone encoding HCC tumor antigen was screened from the cDNA library of Guangxi hepatocellular carcinoma using patient’s own serum, and HCC tumor antigens were tested with serum from patients with liver cancer and others. The specificity of liver cancer, determination of the antigen DNA sequence and check with the gene bank to find its homologous structure. Results: One of the HCC tumor antigens and homozygous marker protein-30 (SMP-30) are homologous structures. The corresponding antibodies were mainly found in HCC patients. The corresponding antibodies were not found in 20 cases of 5 other malignant tumors and 4 cases of liver cirrhosis. Only 20 cases of chronic hepatitis patients and 20 cases of normal human sera showed positive reactions. Conclusion: The senescence marker protein-30 is a newly discovered HCC-associated antigen and has diagnostic significance for HCC.