用含ＮＫＮＤ四个氨基酸序列的特异性ＤＮＡ探针筛选恶性疟原虫ｇｅｎｏｍｉｃＤＮＡ基因文库。阳性克隆的插入片段经ＰＣＲ扩增，限制性内切酶消化后重组进Ｍ１３噬菌体并进行序列测定和分析。获得６个阳性克隆，其插入片段４５０ｂｐ～１．３ｋｂ之间。其中６６１克隆的插入片段为４７７个碱基序列，其一端１２０ｂｐ与探针序列一端完全重叠。表明可合并组成一段含启动子、起始密码子和ＮＫＮＤ四个氨基酸的６７８个碱基的单克隆抗体Ｍ２６～３２抗原决定簇基因片段。 Plasmodium falciparum genomic DNA gene library was screened with a specific DNA probe containing four amino acid sequences of NKND. The positive cloned insert was amplified by PCR, digested with restriction endonuclease, then recombined into M13 phage and sequenced and analyzed. Six positive clones were obtained, which inserted between 450bp and 1.3kb. The insert of 661 clones was 477 base pairs, with 120bp at one end completely overlapping with one end of the probe sequence. This indicates that a 678-base monoclonal antibody M26-32 epitope fragment comprising the four amino acids of the promoter, start codon and NKND can be combined.